Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ATP | half-maximal activation at 0.16 mM ATP | Methanosarcina barkeri | |
additional information | reductive activation by a mixture of H2, ferredoxin needed in the reductive activation process | Methanosarcina barkeri | |
additional information | reductive activation by a mixture of H2, ferredoxin and hydrogenase but also by CO, ATP, GTP or CTP needed in the reductive activation process | Methanosarcina barkeri |
General Stability | Organism |
---|---|
presence of divalent cations e.g. Mg2+, Mn2+, Sr2+, Ca2+ or Ba2+ are required for stability | Methanosarcina barkeri |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
HgCl2 | 1 mM, 90% inhibition | Methanosarcina barkeri | |
Na2SO3 | 1 mM, 50% inhibition | Methanosarcina barkeri | |
NaNO2 | 0.1 mM, 90% inhibition | Methanosarcina barkeri | |
NH4Cl | 400 mM, 93% inhibition | Methanosarcina barkeri | |
O2 | inactivation by O2 and other oxidizing agents | Methanosarcina barkeri | |
pyridoxal 5'-phosphate | 1 mM, 74% inhibition | Methanosarcina barkeri |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | metal-free enzyme preparation has no activity, addition of Ba2+ restores 27% of the original activity | Methanosarcina barkeri | |
Ca2+ | metal-free enzyme preparation has no activity, addition of Ca2+ restores 35% of the original activity | Methanosarcina barkeri | |
Co2+ | metal-free enzyme preparation has no activity, addition of Co+ restores 46% of the original activity | Methanosarcina barkeri | |
Mg2+ | metal-free enzyme preparation has no activity, addition of Mg2+ restores 66% of the original activity | Methanosarcina barkeri | |
Mn2+ | metal-free enzyme preparation has no activity, addition of Mn2+ restores 27% of the original activity | Methanosarcina barkeri | |
Ni2+ | metal-free enzyme preparation has no activity, addition of Ni2+ restores 21% of the original activity | Methanosarcina barkeri | |
Sr2+ | metal-free enzyme preparation has no activity, addition of Sr2+ restores 20% of the original activity | Methanosarcina barkeri |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
alpha2,beta, 2 * 34000 + 1 * 53000, SDS-PAGE in presence of 2-mercaptoethanol | Methanosarcina barkeri |
53000 | - |
alpha2,beta, 2 * 34000 + 1 * 53000, SDS-PAGE in presence of 2-mercaptoethanol | Methanosarcina barkeri |
122000 | - |
PAGE with different gel concentrations | Methanosarcina barkeri |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
methanol + 5-hydroxybenzimidazolylcobamide | Methanosarcina barkeri | one of the enzymes responsible for the transmethylation from methanol to coenzyme M | Co-methyl-Co-5-hydroxybenzimidazolylcobamide + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanosarcina barkeri | Q46EH3 | - |
- |
Oxidation Stability | Organism |
---|---|
oxygen-sensitive | Methanosarcina barkeri |
Purification (Comment) | Organism |
---|---|
- |
Methanosarcina barkeri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methanol + 5-hydroxybenzimidazolylcobamide | - |
Methanosarcina barkeri | Co-methyl-Co-5-hydroxybenzimidazolylcobamide + H2O | - |
? | |
methanol + 5-hydroxybenzimidazolylcobamide | one of the enzymes responsible for the transmethylation from methanol to coenzyme M | Methanosarcina barkeri | Co-methyl-Co-5-hydroxybenzimidazolylcobamide + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | alpha2,beta, 2 * 34000 + 1 * 53000, SDS-PAGE in presence of 2-mercaptoethanol | Methanosarcina barkeri |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Methanosarcina barkeri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Methanosarcina barkeri |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
5-hydroxybenzimidazolylcobamide | 3-4 molecules bound, acting as methyl acceptor | Methanosarcina barkeri |