BRENDA - Enzyme Database
show all sequences of 2.1.1.90

Purification and properties of methanol:5-hydroxybenzimidazolylcobamide methyltransferase from Methanosarcina barkeri

Van der Meijden, P.; te Brömmelstroet, B.W.; Poirot, C.M.; van der Drift, C.; Vogels, G.D.; J. Bacteriol. 160, 629-635 (1984)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
ATP
half-maximal activation at 0.16 mM ATP
Methanosarcina barkeri
additional information
reductive activation by a mixture of H2, ferredoxin and hydrogenase but also by CO, ATP, GTP or CTP needed in the reductive activation process; reductive activation by a mixture of H2, ferredoxin needed in the reductive activation process
Methanosarcina barkeri
General Stability
General Stability
Organism
presence of divalent cations e.g. Mg2+, Mn2+, Sr2+, Ca2+ or Ba2+ are required for stability
Methanosarcina barkeri
Inhibitors
Inhibitors
Commentary
Organism
Structure
HgCl2
1 mM, 90% inhibition
Methanosarcina barkeri
Na2SO3
1 mM, 50% inhibition
Methanosarcina barkeri
NaNO2
0.1 mM, 90% inhibition
Methanosarcina barkeri
NH4Cl
400 mM, 93% inhibition
Methanosarcina barkeri
O2
inactivation by O2 and other oxidizing agents
Methanosarcina barkeri
pyridoxal 5'-phosphate
1 mM, 74% inhibition
Methanosarcina barkeri
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ba2+
metal-free enzyme preparation has no activity, addition of Ba2+ restores 27% of the original activity
Methanosarcina barkeri
Ca2+
metal-free enzyme preparation has no activity, addition of Ca2+ restores 35% of the original activity
Methanosarcina barkeri
Co2+
metal-free enzyme preparation has no activity, addition of Co+ restores 46% of the original activity
Methanosarcina barkeri
Mg2+
metal-free enzyme preparation has no activity, addition of Mg2+ restores 66% of the original activity
Methanosarcina barkeri
Mn2+
metal-free enzyme preparation has no activity, addition of Mn2+ restores 27% of the original activity
Methanosarcina barkeri
Ni2+
metal-free enzyme preparation has no activity, addition of Ni2+ restores 21% of the original activity
Methanosarcina barkeri
Sr2+
metal-free enzyme preparation has no activity, addition of Sr2+ restores 20% of the original activity
Methanosarcina barkeri
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
34000
-
alpha2,beta, 2 * 34000 + 1 * 53000, SDS-PAGE in presence of 2-mercaptoethanol
Methanosarcina barkeri
53000
-
alpha2,beta, 2 * 34000 + 1 * 53000, SDS-PAGE in presence of 2-mercaptoethanol
Methanosarcina barkeri
122000
-
PAGE with different gel concentrations
Methanosarcina barkeri
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
methanol + 5-hydroxybenzimidazolylcobamide
Methanosarcina barkeri
one of the enzymes responsible for the transmethylation from methanol to coenzyme M
Co-methyl-Co-5-hydroxybenzimidazolylcobamide + H2O
-
Methanosarcina barkeri
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Methanosarcina barkeri
Q46EH3
-
-
Oxidation Stability
Oxidation Stability
Organism
oxygen-sensitive
Methanosarcina barkeri
Purification (Commentary)
Commentary
Organism
-
Methanosarcina barkeri
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
methanol + 5-hydroxybenzimidazolylcobamide
-
485660
Methanosarcina barkeri
Co-methyl-Co-5-hydroxybenzimidazolylcobamide + H2O
-
485660
Methanosarcina barkeri
?
methanol + 5-hydroxybenzimidazolylcobamide
one of the enzymes responsible for the transmethylation from methanol to coenzyme M
485660
Methanosarcina barkeri
Co-methyl-Co-5-hydroxybenzimidazolylcobamide + H2O
-
485660
Methanosarcina barkeri
?
Subunits
Subunits
Commentary
Organism
trimer
alpha2,beta, 2 * 34000 + 1 * 53000, SDS-PAGE in presence of 2-mercaptoethanol
Methanosarcina barkeri
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Methanosarcina barkeri
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.2
-
assay at
Methanosarcina barkeri
Cofactor
Cofactor
Commentary
Organism
Structure
5-hydroxybenzimidazolylcobamide
3-4 molecules bound, acting as methyl acceptor
Methanosarcina barkeri
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
ATP
half-maximal activation at 0.16 mM ATP
Methanosarcina barkeri
additional information
reductive activation by a mixture of H2, ferredoxin and hydrogenase but also by CO, ATP, GTP or CTP needed in the reductive activation process; reductive activation by a mixture of H2, ferredoxin needed in the reductive activation process
Methanosarcina barkeri
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
5-hydroxybenzimidazolylcobamide
3-4 molecules bound, acting as methyl acceptor
Methanosarcina barkeri
General Stability (protein specific)
General Stability
Organism
presence of divalent cations e.g. Mg2+, Mn2+, Sr2+, Ca2+ or Ba2+ are required for stability
Methanosarcina barkeri
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
HgCl2
1 mM, 90% inhibition
Methanosarcina barkeri
Na2SO3
1 mM, 50% inhibition
Methanosarcina barkeri
NaNO2
0.1 mM, 90% inhibition
Methanosarcina barkeri
NH4Cl
400 mM, 93% inhibition
Methanosarcina barkeri
O2
inactivation by O2 and other oxidizing agents
Methanosarcina barkeri
pyridoxal 5'-phosphate
1 mM, 74% inhibition
Methanosarcina barkeri
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ba2+
metal-free enzyme preparation has no activity, addition of Ba2+ restores 27% of the original activity
Methanosarcina barkeri
Ca2+
metal-free enzyme preparation has no activity, addition of Ca2+ restores 35% of the original activity
Methanosarcina barkeri
Co2+
metal-free enzyme preparation has no activity, addition of Co+ restores 46% of the original activity
Methanosarcina barkeri
Mg2+
metal-free enzyme preparation has no activity, addition of Mg2+ restores 66% of the original activity
Methanosarcina barkeri
Mn2+
metal-free enzyme preparation has no activity, addition of Mn2+ restores 27% of the original activity
Methanosarcina barkeri
Ni2+
metal-free enzyme preparation has no activity, addition of Ni2+ restores 21% of the original activity
Methanosarcina barkeri
Sr2+
metal-free enzyme preparation has no activity, addition of Sr2+ restores 20% of the original activity
Methanosarcina barkeri
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
34000
-
alpha2,beta, 2 * 34000 + 1 * 53000, SDS-PAGE in presence of 2-mercaptoethanol
Methanosarcina barkeri
53000
-
alpha2,beta, 2 * 34000 + 1 * 53000, SDS-PAGE in presence of 2-mercaptoethanol
Methanosarcina barkeri
122000
-
PAGE with different gel concentrations
Methanosarcina barkeri
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
methanol + 5-hydroxybenzimidazolylcobamide
Methanosarcina barkeri
one of the enzymes responsible for the transmethylation from methanol to coenzyme M
Co-methyl-Co-5-hydroxybenzimidazolylcobamide + H2O
-
Methanosarcina barkeri
?
Oxidation Stability (protein specific)
Oxidation Stability
Organism
oxygen-sensitive
Methanosarcina barkeri
Purification (Commentary) (protein specific)
Commentary
Organism
-
Methanosarcina barkeri
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
methanol + 5-hydroxybenzimidazolylcobamide
-
485660
Methanosarcina barkeri
Co-methyl-Co-5-hydroxybenzimidazolylcobamide + H2O
-
485660
Methanosarcina barkeri
?
methanol + 5-hydroxybenzimidazolylcobamide
one of the enzymes responsible for the transmethylation from methanol to coenzyme M
485660
Methanosarcina barkeri
Co-methyl-Co-5-hydroxybenzimidazolylcobamide + H2O
-
485660
Methanosarcina barkeri
?
Subunits (protein specific)
Subunits
Commentary
Organism
trimer
alpha2,beta, 2 * 34000 + 1 * 53000, SDS-PAGE in presence of 2-mercaptoethanol
Methanosarcina barkeri
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Methanosarcina barkeri
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.2
-
assay at
Methanosarcina barkeri
Other publictions for EC 2.1.1.90
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
726582
Hoeppner
Structure of the corrinoid:coe ...
Methanosarcina mazei
Acta Crystallogr. Sect. D
68
1549-1557
2012
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698630
Opulencia
Physiology and posttranscripti ...
Methanosarcina acetivorans
J. Bacteriol.
191
6928-6935
2009
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680470
Bose
Differential regulation of the ...
Methanosarcina acetivorans
J. Bacteriol.
188
7274-7283
2006
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682524
Hagemeier
Insight into the mechanism of ...
Methanosarcina barkeri
Proc. Natl. Acad. Sci. USA
103
18917-18922
2006
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681980
Pritchett
Genetic, physiological and bio ...
Methanosarcina acetivorans
Mol. Microbiol.
56
1183-1194
2005
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485665
Sauer
Methanol:coenzyme M methyltran ...
Methanosarcina barkeri
Eur. J. Biochem.
249
280-285
1997
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485666
Sauer
Methanol:coenzyme M methyltran ...
Methanosarcina barkeri
Eur. J. Biochem.
243
670-677
1997
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727819
Daas
Activation mechanism of methan ...
Methanosarcina barkeri, Methanosarcina barkeri DSM 800
J. Biol. Chem.
271
22346-2251
1996
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485660
Van der Meijden
Purification and properties of ...
Methanosarcina barkeri
J. Bacteriol.
160
629-635
1984
2
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1
6
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3
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485661
Van der Meijden
Reductive activation of methan ...
Methanosarcina barkeri
Biochem. Biophys. Res. Commun.
118
760-766
1984
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485663
Van der Meijden
-
Methanol conversion in Eubacte ...
Eubacterium limosum, Methanothermobacter thermautotrophicus
Arch. Microbiol.
138
360-364
1984
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485662
Van der Meijden
Methyltransferases involved in ...
Methanosarcina barkeri
Arch. Microbiol.
134
238-242
1983
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485664
Taylor
A simplified assay for coenzym ...
Methanobacterium bryantii
J. Biol. Chem.
249
4886-4890
1974
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