Literature summary for 2.1.1.85 extracted from

Wilkinson, A.W.; Diep, J.; Dai, S.; Liu, S.; Ooi, Y.S.; Song, D.; Li, T.M.; Horton, J.R.; Zhang, X.; Liu, C.; Trivedi, D.V.; Ruppel, K.M.; Vilches-Moure, J.G.; Casey, K.M.; Mak, J.; Cowan, T.; Elias, J.E.; Nagamine, C.M.; Spudich, J.A.; Cheng, X.; Carette, J.E.; Gozani, O.
SETD3 is an actin histidine methyltransferase that prevents primary dystocia (2019), Nature, 565, 372-376 .

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21 or BL21(DE3) Codon-plus cells	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
enzyme in complex with an actin peptide (residues 66-80) that contains His73 in the presence of S-adenosyl-L-homocysteine, hanging drop vapor diffusion method, using either 0.2 M ammonium acetate, 0.1 M sodium citrate tribasic dihydrate pH 5.6 and 30% (w/v) polyethylene glycol 4000, or 0.2 M ammonium sulfate or 0.2 M sodium chloride, 0.1 M Bis-Tris pH 5.5, 25% (w/v) polyethylene glycol 3350 and 0.1 M Bis-Tris pH 5.5, 25% (w/v) polyethylene glycol 3350	Homo sapiens

Crystallization (Comment)

Organism

enzyme in complex with an actin peptide (residues 66-80) that contains His73 in the presence of S-adenosyl-L-homocysteine, hanging drop vapor diffusion method, using either 0.2 M ammonium acetate, 0.1 M sodium citrate tribasic dihydrate pH 5.6 and 30% (w/v) polyethylene glycol 4000, or 0.2 M ammonium sulfate or 0.2 M sodium chloride, 0.1 M Bis-Tris pH 5.5, 25% (w/v) polyethylene glycol 3350 and 0.1 M Bis-Tris pH 5.5, 25% (w/v) polyethylene glycol 3350

Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
Y312A	catalytical mutant with residual activity	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
nucleosome	-	Mus musculus	786	-
nucleosome	-	Homo sapiens	786	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
S-adenosyl-L-methionine + actin L-histidine73	Mus musculus	-	S-adenosyl-L-homocysteine + actin Ntau-methyl-L-histidine73	-	?
S-adenosyl-L-methionine + actin L-histidine73	Homo sapiens	-	S-adenosyl-L-homocysteine + actin Ntau-methyl-L-histidine73	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q86TU7	-	-
Mus musculus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
glutathione Sepharose column chromatography and Ni-NTA agarose column chromatography	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
HT-1080 cell	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
additional information	the enzyme does not methylate histones and shows no activity on actin I71A/W79E double mutations	Homo sapiens	?	-	-
S-adenosyl-L-methionine + actin L-histidine73	-	Mus musculus	S-adenosyl-L-homocysteine + actin Ntau-methyl-L-histidine73	-	?
S-adenosyl-L-methionine + actin L-histidine73	-	Homo sapiens	S-adenosyl-L-homocysteine + actin Ntau-methyl-L-histidine73	-	?
S-adenosyl-L-methionine + human beta-actin (residues 66-80)	sequence TLKYPIEHGIVTNWD	Homo sapiens	S-adenosyl-L-homocysteine + human beta-actin (residues 66-80) Ntau-methyl-L-histidine	-	?

Subunits

Subunits	Comment	Organism
?	x * 80000, SDS-PAGE	Mus musculus
?	x * 80000, SDS-PAGE	Homo sapiens

Synonyms

Synonyms	Comment	Organism
actin His73 nmethyltransferase	-	Mus musculus
actin His73 nmethyltransferase	-	Homo sapiens
actin histidine methyltransferase	-	Mus musculus
actin histidine methyltransferase	-	Homo sapiens
SET domain protein 3	-	Mus musculus
SET domain protein 3	-	Homo sapiens
setd3	-	Mus musculus
setd3	-	Homo sapiens

General Information

General Information	Comment	Organism
malfunction	enzyme depletion impairs signal-induced contraction in primary uterine smooth muscle cells	Homo sapiens
malfunction	enzyme-deficient female mice have severely decreased litter sizes owing to primary maternal dystocia that is refractory to ecbolic induction agents	Mus musculus