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Literature summary for 2.1.1.85 extracted from

  • Guo, Q.; Liao, S.; Kwiatkowski, S.; Tomaka, W.; Yu, H.; Wu, G.; Tu, X.; Min, J.; Drozak, J.; Xu, C.
    Structural insights into SETD3-mediated histidine methylation on beta-actin (2019), eLife, 8, e43676 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.85

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
S-adenosyl-L-homocysteine-bound enzyme in complex with either an unmodified beta-actin peptide or its His-methylated variant, sitting drop vapor diffusion method, using 0.1 M sodium cacodylate trihydrate (pH 6.5), 0.2 M magnesium acetate tetrahydrate, and 20% (w/v) polyethylene glycol 8000 Homo sapiens

Protein Variants

Protein Variants Comment Organism
N256A the mutant shows severely reduced activity compared to the wild type enzyme Homo sapiens
N256D the mutant shows severely reduced activity compared to the wild type enzyme Homo sapiens
N256Q the mutant shows about 50% activity compared to the wild type enzyme Homo sapiens
N278A the mutant shows severely reduced activity compared to the wild type enzyme Homo sapiens
R215A the mutant shows severely reduced activity compared to the wild type enzyme Homo sapiens
R316A the mutant shows severely reduced activity compared to the wild type enzyme Homo sapiens
R75A the mutant shows severely reduced activity compared to the wild type enzyme Homo sapiens
Y313F the mutant shows severely reduced activity compared to the wild type enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000047
-
 beta-actin L-histidine73 mutant enzyme N278A, at pH 7.2 and 37°C Homo sapiens
0.000103
-
 S-adenosyl-L-methionine mutant enzyme N256Q, at pH 7.2 and 37°C Homo sapiens
0.000111
-
 S-adenosyl-L-methionine wild type enzyme, at pH 7.2 and 37°C Homo sapiens
0.000149
-
 S-adenosyl-L-methionine mutant enzyme R316A, at pH 7.2 and 37°C Homo sapiens
0.000165
-
 S-adenosyl-L-methionine mutant enzyme N256D, at pH 7.2 and 37°C Homo sapiens
0.000165
-
 S-adenosyl-L-methionine mutant enzyme R215A, at pH 7.2 and 37°C Homo sapiens
0.000305
-
 beta-actin L-histidine73 mutant enzyme N256D, at pH 7.2 and 37°C Homo sapiens
0.00035
-
 S-adenosyl-L-methionine mutant enzyme N278A, at pH 7.2 and 37°C Homo sapiens
0.000449
-
 beta-actin L-histidine73 mutant enzyme N256A, at pH 7.2 and 37°C Homo sapiens
0.000502
-
 beta-actin L-histidine73 wild type enzyme, at pH 7.2 and 37°C Homo sapiens
0.000565
-
 beta-actin L-histidine73 mutant enzyme R75A, at pH 7.2 and 37°C Homo sapiens
0.000581
-
 beta-actin L-histidine73 mutant enzyme Y313F, at pH 7.2 and 37°C Homo sapiens
0.000686
-
 S-adenosyl-L-methionine mutant enzyme N256A, at pH 7.2 and 37°C Homo sapiens
0.000784
-
 beta-actin L-histidine73 mutant enzyme N256Q, at pH 7.2 and 37°C Homo sapiens
0.001087
-
 beta-actin L-histidine73 mutant enzyme R215A, at pH 7.2 and 37°C Homo sapiens
0.001115
-
 beta-actin L-histidine73 mutant enzyme R316A, at pH 7.2 and 37°C Homo sapiens
0.003601
-
 S-adenosyl-L-methionine mutant enzyme R75A, at pH 7.2 and 37°C Homo sapiens
0.006543
-
 S-adenosyl-L-methionine mutant enzyme Y313F, at pH 7.2 and 37°C Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + beta-actin L-histidine73 Homo sapiens
-
 S-adenosyl-L-homocysteine + beta-actin Ntau-methyl-L-histidine73
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q86TU7
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and HisTrap column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with histone H3 L-lysine4 (1-23), histone H3 L-lysine36 (25-47), and beta-actin (66-88) H73A mutant Homo sapiens ?
-
-
S-adenosyl-L-methionine + beta-actin L-histidine73
-
 Homo sapiens S-adenosyl-L-homocysteine + beta-actin Ntau-methyl-L-histidine73
-
 ?
S-adenosyl-L-methionine + synthetic peptide corresponding to residue 66-88 of beta-actin
-
 Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
actin-specific methyltransferase
-
 Homo sapiens
setd3
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.000017
-
 S-adenosyl-L-methionine mutant enzyme N278A, at pH 7.2 and 37°C Homo sapiens
0.000017
-
 beta-actin L-histidine73 mutant enzyme N278A, at pH 7.2 and 37°C Homo sapiens
0.000033
-
 beta-actin L-histidine73 mutant enzyme R75A, at pH 7.2 and 37°C Homo sapiens
0.00027
-
 S-adenosyl-L-methionine mutant enzyme R75A, at pH 7.2 and 37°C Homo sapiens
0.00037
-
 beta-actin L-histidine73 mutant enzyme Y313F, at pH 7.2 and 37°C Homo sapiens
0.00052
-
 beta-actin L-histidine73 mutant enzyme N256A, at pH 7.2 and 37°C Homo sapiens
0.00067
-
 S-adenosyl-L-methionine mutant enzyme N256D, at pH 7.2 and 37°C Homo sapiens
0.00097
-
 beta-actin L-histidine73 mutant enzyme N256D, at pH 7.2 and 37°C Homo sapiens
0.0011
-
 S-adenosyl-L-methionine mutant enzyme N256A, at pH 7.2 and 37°C Homo sapiens
0.0016
-
 S-adenosyl-L-methionine mutant enzyme Y313F, at pH 7.2 and 37°C Homo sapiens
0.0029
-
 beta-actin L-histidine73 mutant enzyme R316A, at pH 7.2 and 37°C Homo sapiens
0.0035
-
 beta-actin L-histidine73 mutant enzyme R215A, at pH 7.2 and 37°C Homo sapiens
0.00365
-
 S-adenosyl-L-methionine mutant enzyme R215A, at pH 7.2 and 37°C Homo sapiens
0.0041
-
 S-adenosyl-L-methionine mutant enzyme R316A, at pH 7.2 and 37°C Homo sapiens
0.0063
-
 S-adenosyl-L-methionine mutant enzyme N256Q, at pH 7.2 and 37°C Homo sapiens
0.011
-
 beta-actin L-histidine73 mutant enzyme N256Q, at pH 7.2 and 37°C Homo sapiens
0.0112
-
 S-adenosyl-L-methionine wild type enzyme, at pH 7.2 and 37°C Homo sapiens
0.013
-
 beta-actin L-histidine73 wild type enzyme, at pH 7.2 and 37°C Homo sapiens

General Information

General Information Comment Organism
physiological function the enzyme plays important roles in hypoxic pulmonary hypertension, muscle differentiation, and carcinogenesis Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.05
-
 S-adenosyl-L-methionine mutant enzyme N278A, at pH 7.2 and 37°C Homo sapiens
0.067
-
 S-adenosyl-L-methionine mutant enzyme R75A, at pH 7.2 and 37°C Homo sapiens
0.067
-
 beta-actin L-histidine73 mutant enzyme R75A, at pH 7.2 and 37°C Homo sapiens
0.25
-
 S-adenosyl-L-methionine mutant enzyme Y313F, at pH 7.2 and 37°C Homo sapiens
0.35
-
 beta-actin L-histidine73 mutant enzyme N278A, at pH 7.2 and 37°C Homo sapiens
0.63
-
 beta-actin L-histidine73 mutant enzyme Y313F, at pH 7.2 and 37°C Homo sapiens
1.15
-
 beta-actin L-histidine73 mutant enzyme N256A, at pH 7.2 and 37°C Homo sapiens
1.65
-
 S-adenosyl-L-methionine mutant enzyme N256A, at pH 7.2 and 37°C Homo sapiens
2.58
-
 beta-actin L-histidine73 mutant enzyme R316A, at pH 7.2 and 37°C Homo sapiens
3.17
-
 beta-actin L-histidine73 mutant enzyme N256D, at pH 7.2 and 37°C Homo sapiens
3.25
-
 beta-actin L-histidine73 mutant enzyme R215A, at pH 7.2 and 37°C Homo sapiens
4.03
-
 S-adenosyl-L-methionine mutant enzyme N256D, at pH 7.2 and 37°C Homo sapiens
13.8
-
 beta-actin L-histidine73 mutant enzyme N256Q, at pH 7.2 and 37°C Homo sapiens
22.12
-
 S-adenosyl-L-methionine mutant enzyme R215A, at pH 7.2 and 37°C Homo sapiens
26.87
-
 beta-actin L-histidine73 wild type enzyme, at pH 7.2 and 37°C Homo sapiens
27.3
-
 S-adenosyl-L-methionine mutant enzyme R316A, at pH 7.2 and 37°C Homo sapiens
61.48
-
 S-adenosyl-L-methionine mutant enzyme N256Q, at pH 7.2 and 37°C Homo sapiens
101.05
-
 S-adenosyl-L-methionine wild type enzyme, at pH 7.2 and 37°C Homo sapiens