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Literature summary for 2.1.1.79 extracted from

  • Ma, Y.; Pan, C.; Wang, Q.
    Crystal structure of bacterial cyclopropane-fatty-acyl-phospholipid synthase with phospholipid (2019), J. Biochem., 166, 139-147 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Lactobacillus acidophilus

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 2 M ammonium sulfate, 0.2M potassium sodium tartrate tetrahydrate and 0.1 M sodium citrate tribasic dehydrate, pH 5.6 Lactobacillus acidophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.092
-
S-adenosyl-L-methionine with Tris/NaHCO3, at pH 8.0 and 37°C Lactobacillus acidophilus
0.113
-
S-adenosyl-L-methionine with Tris, at pH 8.0 and 37°C Lactobacillus acidophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + phospholipid olefinic fatty acid Lactobacillus acidophilus
-
S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
-
?
S-adenosyl-L-methionine + phospholipid olefinic fatty acid Lactobacillus acidophilus NCFM
-
S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
-
?

Organism

Organism UniProt Comment Textmining
Lactobacillus acidophilus Q5FJM0
-
-
Lactobacillus acidophilus NCFM Q5FJM0
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Superdex S200 gel filtration Lactobacillus acidophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + phospholipid olefinic fatty acid
-
Lactobacillus acidophilus S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
-
?
S-adenosyl-L-methionine + phospholipid olefinic fatty acid
-
Lactobacillus acidophilus NCFM S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
-
?

Subunits

Subunits Comment Organism
? x * 46000, SDS-PAGE Lactobacillus acidophilus

Synonyms

Synonyms Comment Organism
CFA synthase
-
Lactobacillus acidophilus
CFAS
-
Lactobacillus acidophilus
cyclopropane-fatty-acyl-phospholipid synthase
-
Lactobacillus acidophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.048
-
S-adenosyl-L-methionine with Tris, at pH 8.0 and 37°C Lactobacillus acidophilus
0.071
-
S-adenosyl-L-methionine with Tris/NaHCO3, at pH 8.0 and 37°C Lactobacillus acidophilus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.43
-
S-adenosyl-L-methionine with Tris, at pH 8.0 and 37°C Lactobacillus acidophilus
0.77
-
S-adenosyl-L-methionine with Tris/NaHCO3, at pH 8.0 and 37°C Lactobacillus acidophilus