Activating Compound | Comment | Organism | Structure |
---|---|---|---|
bicarbonate | required. CFA synthase isolated and assayed in potassium bicarbonate buffer displayes more than 3-fold higher activity than in HEPES buffer | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
E239A | mutant shows 0.57% of wild-type activity | Escherichia coli |
E239D | mutant shows 0.96% of wild-type activity | Escherichia coli |
H266A | mutant shows 2.1% of wild-type activity | Escherichia coli |
Y317F | mutant shows 0.45% of wild-type activity | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Borate | competitive | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0705 | - |
S-adenosyl-L-methionine | purification buffer/assay buffer: KHCO3/HEPES | Escherichia coli | |
0.0716 | - |
S-adenosyl-L-methionine | purification buffer/assay buffer: HEPES/KHCO3 | Escherichia coli | |
0.0887 | - |
S-adenosyl-L-methionine | purification buffer/assay buffer: HEPES/HEPES | Escherichia coli | |
0.0997 | - |
S-adenosyl-L-methionine | purification buffer/assay buffer: KHCO3/KHCO3 | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + phospholipid olefinic fatty acid | - |
Escherichia coli | S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CFA synthase | - |
Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
2.02 | - |
Borate | - |
Escherichia coli |