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Literature summary for 2.1.1.79 extracted from
- Escherichia coli cyclopropane fatty acid synthase (2004), Eur. J. Biochem., 271, 4769-4778.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overproduced as a His6-tagged protein | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C139S | 15% of wild-type activity | Escherichia coli |
| C176S | 150% of wild-type activity | Escherichia coli |
| C354S | 63% of wild-type activity | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5,5'-dithiobis-(2-nitrobenzoic acid) | substrate does not protect against inactivation | Escherichia coli |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.073 | - |
S-adenosyl-L-methionine | mutant enzyme C176S | Escherichia coli | |
| 0.088 | - |
S-adenosyl-L-methionine | mutant enzyme C139S | Escherichia coli | |
| 0.09 | - |
S-adenosyl-L-methionine | wild-type enzyme | Escherichia coli | |
| 0.105 | - |
S-adenosyl-L-methionine | mutant enzyme C354S | Escherichia coli | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged protein | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.05 | - |
- |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, pH 7.4, 20 mM phosphate buffer, 50% v/v glycerol, best storage conditions | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CFAS | - |
Escherichia coli |
| cyclopropane fatty acid synthase | - |
Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.3 | - |
S-adenosyl-L-methionine | mutant enzyme C139S | Escherichia coli | |
| 1.6 | - |
S-adenosyl-L-methionine | mutant enzyme C354S | Escherichia coli | |
| 2.2 | - |
S-adenosyl-L-methionine | wild-type enzyme | Escherichia coli | |
| 2.7 | - |
S-adenosyl-L-methionine | mutant enzyme C176S | Escherichia coli | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Escherichia coli |
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Release 2023.1 (January 2023)
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