BRENDA - Enzyme Database
show all sequences of 2.1.1.79

Cyclopropane fatty acid synthetase: partial purification and properties

Chung, A.E.; Law, J.H.; Biochemistry 3, 967-974 (1964)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
sodium lauryl sulfate
stimulates
Clostridium butyricum
Inhibitors
Inhibitors
Commentary
Organism
Structure
Cutsum
-
Clostridium butyricum
hexadecyltrimethylammonium bromide
-
Clostridium butyricum
phosphatidylcholine
-
Clostridium butyricum
Tween 80
-
Clostridium butyricum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.057
-
S-adenosyl-L-methionine
-
Clostridium butyricum
0.53
-
phosphatidylethanolamine
-
Clostridium butyricum
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Clostridium butyricum
-
-
-
Purification (Commentary)
Commentary
Organism
partial
Clostridium butyricum
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Clostridium butyricum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-homocysteine + phosphatidylcholine
phosphatidylcholine is not a substrate
485625
Clostridium butyricum
S-adenosyl-L-homocysteine + ?
-
-
-
-
S-adenosyl-L-methionine + phosphatidylethanolamine
-
485625
Clostridium butyricum
S-adenosyl-L-homocysteine + ?
-
-
-
-
S-adenosyl-L-methionine + phospholipid olefinic fatty acid
-
485625
Clostridium butyricum
S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
-
485625
Clostridium butyricum
?
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
sodium lauryl sulfate
stimulates
Clostridium butyricum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Cutsum
-
Clostridium butyricum
hexadecyltrimethylammonium bromide
-
Clostridium butyricum
phosphatidylcholine
-
Clostridium butyricum
Tween 80
-
Clostridium butyricum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.057
-
S-adenosyl-L-methionine
-
Clostridium butyricum
0.53
-
phosphatidylethanolamine
-
Clostridium butyricum
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Clostridium butyricum
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Clostridium butyricum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-homocysteine + phosphatidylcholine
phosphatidylcholine is not a substrate
485625
Clostridium butyricum
S-adenosyl-L-homocysteine + ?
-
-
-
-
S-adenosyl-L-methionine + phosphatidylethanolamine
-
485625
Clostridium butyricum
S-adenosyl-L-homocysteine + ?
-
-
-
-
S-adenosyl-L-methionine + phospholipid olefinic fatty acid
-
485625
Clostridium butyricum
S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
-
485625
Clostridium butyricum
?
Other publictions for EC 2.1.1.79
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
733222
To
Cyclopropane fatty acid syntha ...
Oenococcus oeni
Arch. Microbiol.
197
1063-1074
2015
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734414
Broadbent
Influence of polysorbate 80 an ...
Lactobacillus casei, Lactobacillus casei ATCC 334
J. Ind. Microbiol. Biotechnol.
41
545-553
2014
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1
1
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734968
Yu
Coexpressing Escherichia coli ...
Escherichia coli
Plant Physiol.
164
455-465
2014
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3
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733434
E
An active site mutant of Esche ...
Escherichia coli
Biochimie
95
2336-2344
2013
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1
-
1
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-
-
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1
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4
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1
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2
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2
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1
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-
-
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-
720395
Palacios-Chaves
Identification and functional ...
Brucella abortus, Brucella abortus 2308 NalR
Microbiology
158
1037-1044
2012
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10
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2
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2
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1
2
2
1
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-
735077
Oyola
Functional analysis of Leishma ...
Leishmania infantum
PLoS ONE
7
e51300
2012
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1
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1
1
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6
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1
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1
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1
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1
1
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1
1
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-
719184
Yu
Characterization and analysis ...
Gossypium hirsutum
BMC Plant Biol.
11
97
2011
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1
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1
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6
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5
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5
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1
1
1
1
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719577
Jones
Cyclopropane fatty acid syntha ...
Lactobacillus reuteri, Lactobacillus reuteri ATCC PTA 6475
Gut Microbes
2
69-79
2011
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5
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4
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4
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2
2
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719008
Guangqi
Insight into the reaction mech ...
Escherichia coli
Biochimie
92
1454-1457
2010
-
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1
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3
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1
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2
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705574
Saborido Basconcillo
Cyclopropane fatty acyl syntha ...
Sinorhizobium meliloti, Sinorhizobium meliloti RmP110
Microbiology
155
373-385
2009
-
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4
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2
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1
1
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-
685391
Guianvarch
Identification of inhibitors o ...
Escherichia coli
Biochim. Biophys. Acta
1784
1652-1658
2008
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1
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9
2
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2
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1
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1
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1
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8
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9
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1
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9
9
8
2
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1
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1
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1
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705554
Grandvalet
Changes in membrane lipid comp ...
Oenococcus oeni
Microbiology
154
2611-2619
2008
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1
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4
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1
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1
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705771
Rosenthal
Regulation of transcription by ...
Escherichia coli
Mol. Microbiol.
68
907-917
2008
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2
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1
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1
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-
677669
Munoz-Rojas
Involvement of cyclopropane fa ...
Pseudomonas putida, Pseudomonas putida KT 2240
Appl. Environ. Microbiol.
72
472-477
2006
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45
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2
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2
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678402
Guianvarch
Identification of new inhibito ...
Escherichia coli
Biochim. Biophys. Acta
1764
1381-1388
2006
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7
1
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3
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1
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1
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1
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5
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1
7
5
1
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1
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1
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659895
Kim
The formation of cyclopropane ...
Salmonella enterica
Microbiology
151
209-218
2005
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1
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1
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4
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678131
Courtois
Escherichia coli cyclopropane ...
Escherichia coli
Biochemistry
44
13583-13590
2005
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5
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3
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3
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1
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1
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1
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3
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679889
Budin-Verneuil
Transcriptional analysis of th ...
Lactococcus lactis, Lactococcus lactis MG1363
FEMS Microbiol. Lett.
250
189-194
2005
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680343
Iwig
The activity of Escherichia co ...
Escherichia coli
J. Am. Chem. Soc.
127
11612-11613
2005
1
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4
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1
4
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2
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658039
Iwig
Isotope and elemental effects ...
Escherichia coli
Biochemistry
43
13510-13524
2004
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3
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3
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1
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3
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3
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3
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3
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658648
Courtois
Escherichia coli cyclopropane ...
Escherichia coli
Eur. J. Biochem.
271
4769-4778
2004
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1
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3
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1
4
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2
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1
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1
1
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4
1
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1
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3
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4
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1
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1
1
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4
1
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485635
Bao
-
Characterization of cyclopropa ...
Sterculia foetida
J. Biol. Chem.
31
1-36
2003
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2
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1
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1
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1
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2
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3
1
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658447
Molitor
Cyclopropane fatty acid syntha ...
Escherichia coli
ChemBioChem
4
1352-1356
2003
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3
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1
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485634
Bao
Carbocyclic fatty acids in pla ...
Sterculia foetida
Proc. Natl. Acad. Sci. USA
99
7172-7177
2002
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485632
Chang
Metabolic instability of Esche ...
Escherichia coli
J. Bacteriol.
182
4288-4294
2000
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485633
Kuchta
-
Glycinebetaine stimulates, but ...
Eubacterium HX
Arch. Microbiol.
161
234-238
1994
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485627
Wang
Cyclopropane fatty acid syntha ...
Escherichia coli
Biochemistry
31
11020-11028
1992
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485628
Grogan
Cloning and manipulation of th ...
Escherichia coli
J. Bacteriol.
158
286-295
1984
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485626
Taylor
Cyclopropane fatty acid syntha ...
Escherichia coli, Escherichia coli B / ATCC 11303
Methods Enzymol.
71
133-139
1981
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485629
Smith
Inhibition of cyclopropane fat ...
Lactobacillus plantarum
Biochem. Biophys. Res. Commun.
94
1458-1462
1980
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485630
Smith
S-Adenosylmethionine, cyclopro ...
Lactobacillus plantarum
Arch. Biochem. Biophys.
205
564-570
1980
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485631
Taylor
Cyclopropane fatty acid syntha ...
Escherichia coli
Biochemistry
18
3292-3300
1979
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485625
Chung
Cyclopropane fatty acid synthe ...
Clostridium butyricum
Biochemistry
3
967-974
1964
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485624
Zalkin
Enzymic synthesis of cycloprop ...
Clostridium butyricum, Serratia marcescens
J. Biol. Chem.
238
1242-1248
1963
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