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Literature summary for 2.1.1.77 extracted from

  • Kumawat, M.; Pesingi, P.K.; Agarwal, R.K.; Goswami, T.K.; Mahawar, M.
    Contribution of protein isoaspartate methyl transferase (PIMT) in the survival of Salmonella Typhimurium under oxidative stress and virulence (2016), Int. J. Med. Microbiol., 306, 222-230 .
    View publication on PubMed
Search for more literature for 2.1.1.77

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Salmonella enterica subsp. enterica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + protein L-isoaspartate Salmonella enterica subsp. enterica
-
 S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
 ?
S-adenosyl-L-methionine + protein L-isoaspartate Salmonella enterica subsp. enterica E-2375
-
 S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
 ?

Organism

Organism UniProt Comment Textmining
Salmonella enterica subsp. enterica
-
 serovar typhimurium
-
Salmonella enterica subsp. enterica E-2375
-
 serovar typhimurium
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Superdex S200 gel filtration Salmonella enterica subsp. enterica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + protein L-isoaspartate
-
 Salmonella enterica subsp. enterica S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
 ?
S-adenosyl-L-methionine + protein L-isoaspartate
-
 Salmonella enterica subsp. enterica E-2375 S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
 ?

Subunits

Subunits Comment Organism
? x * 23000, SDS-PAGE Salmonella enterica subsp. enterica

Synonyms

Synonyms Comment Organism
PIMT
-
 Salmonella enterica subsp. enterica
protein isoaspartate methyl transferase
-
 Salmonella enterica subsp. enterica

General Information

General Information Comment Organism
malfunction in comparison to wild type, the enzyme deletion strain is found significantly more susceptible to H2O2 (4fold at 2.5 mM) and hypochlorite (90fold at 0.2 mM). Further, the enzyme deletion mutant strain shows hypersusceptibility to interferon-gamma stimulated macrophages Salmonella enterica subsp. enterica
physiological function by repairing iso-Asp to Asp, the enzyme maintains the activities of proteins and thus helps in cellular survival under oxidative stress Salmonella enterica subsp. enterica