Application | Comment | Organism |
---|---|---|
biotechnology | useful in biotechnical applications | Thermotoga maritima |
Cloned (Comment) | Organism |
---|---|
disruption of the pcm gene | Caenorhabditis elegans |
pcm gene PCR amplified, overexpressed in Escherichia coli as a fusion protein | Thermotoga maritima |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00029 | - |
VYP (isoD) HA | - |
Homo sapiens | |
0.00052 | - |
KASA (isoD) LAKY | - |
Homo sapiens | |
0.0019 | - |
S-adenosyl-L-methionine | 37°C | Thermotoga maritima | |
0.002 | - |
S-adenosyl-L-methionine | - |
Homo sapiens | |
0.0028 | - |
KASA (isoD) LAKY | 37°C | Thermotoga maritima | |
0.0031 | - |
S-adenosyl-L-methionine | - |
Caenorhabditis elegans | |
0.0041 | - |
VYP (isoD) HA | 37°C | Thermotoga maritima | |
0.0056 | - |
S-adenosyl-L-methionine | 85°C | Thermotoga maritima | |
0.00912 | - |
KASA (isoD) LAKY | - |
Caenorhabditis elegans | |
0.0118 | - |
VYP (isoD) HA | - |
Escherichia coli | |
0.0127 | - |
KASA (isoD) LAKY | - |
Triticum aestivum | |
0.0194 | - |
VYP (isoD) HA | - |
Caenorhabditis elegans | |
0.0217 | - |
VYP (isoD) HA | - |
Triticum aestivum | |
0.025 | - |
KASA (isoD) LAKY | 85°C | Thermotoga maritima | |
0.0336 | - |
VYP (isoD) HA | 85°C | Thermotoga maritima | |
0.035 | - |
ovalbumin | - |
Homo sapiens | |
0.0506 | - |
KASA (isoD) LAKY | - |
Escherichia coli | |
0.326 | - |
ovalbumin | 37°C | Thermotoga maritima | |
0.727 | - |
ovalbumin | - |
Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Mus musculus | 5829 | - |
cytosol | - |
Escherichia coli | 5829 | - |
cytosol | - |
Homo sapiens | 5829 | - |
cytosol | - |
Rattus norvegicus | 5829 | - |
cytosol | - |
Caenorhabditis elegans | 5829 | - |
cytosol | - |
Thermotoga maritima | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
1 * 34000, equilibrium centrifugation | Thermotoga maritima |
35000 | - |
SDS-PAGE | Escherichia coli |
36400 | - |
calculated from translated amino acid sequence | Thermotoga maritima |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + protein L-isoaspartate | Mus musculus | - |
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester | - |
? | |
S-adenosyl-L-methionine + protein L-isoaspartate | Escherichia coli | - |
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester | - |
? | |
S-adenosyl-L-methionine + protein L-isoaspartate | Homo sapiens | - |
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester | - |
? | |
S-adenosyl-L-methionine + protein L-isoaspartate | Rattus norvegicus | - |
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester | - |
? | |
S-adenosyl-L-methionine + protein L-isoaspartate | Triticum aestivum | - |
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester | - |
? | |
S-adenosyl-L-methionine + protein L-isoaspartate | Methanothermobacter thermautotrophicus | - |
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester | - |
? | |
S-adenosyl-L-methionine + protein L-isoaspartate | Caenorhabditis elegans | - |
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester | - |
? | |
S-adenosyl-L-methionine + protein L-isoaspartate | Methanocaldococcus jannaschii | - |
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester | - |
? | |
S-adenosyl-L-methionine + protein L-isoaspartate | Archaeoglobus fulgidus | - |
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester | - |
? | |
S-adenosyl-L-methionine + protein L-isoaspartate | Thermotoga maritima | highly active protein repair enzyme | S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Archaeoglobus fulgidus | - |
- |
- |
Caenorhabditis elegans | - |
- |
- |
Escherichia coli | - |
- |
- |
Homo sapiens | - |
human | - |
Methanocaldococcus jannaschii | - |
- |
- |
Methanothermobacter thermautotrophicus | - |
- |
- |
Mus musculus | - |
- |
- |
no activity in Aquifex aeolicus | - |
- |
- |
no activity in Bacillus subtilis | - |
- |
- |
no activity in Borrelia burgdorferi | - |
- |
- |
no activity in Haemophilus influenzae | - |
- |
- |
no activity in Mycoplasma genitalium | - |
- |
- |
no activity in Mycoplasma pneumoniae | - |
- |
- |
no activity in Sinorhizobium meliloti | - |
- |
- |
no activity in Treponema pallidum | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Thermotoga maritima | - |
- |
- |
Triticum aestivum | - |
wheat | - |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
- |
Homo sapiens |
- |
Triticum aestivum |
- |
Thermotoga maritima |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
erythrocyte | - |
Mus musculus | - |
erythrocyte | - |
Homo sapiens | - |
erythrocyte | - |
Rattus norvegicus | - |
germ | - |
Triticum aestivum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0022 | - |
37°C | Thermotoga maritima |
0.156 | - |
- |
Thermotoga maritima |
Storage Stability | Organism |
---|---|
-80°C, stored in liquid nitrogen, no apparent loss of activity | Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + KASA (isoD) LAKY | synthetic methyl acceptor | Escherichia coli | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + KASA (isoD) LAKY | synthetic methyl acceptor | Homo sapiens | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + KASA (isoD) LAKY | synthetic methyl acceptor | Triticum aestivum | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + KASA (isoD) LAKY | synthetic methyl acceptor | Caenorhabditis elegans | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + KASA (isoD) LAKY | synthetic methyl acceptor | Thermotoga maritima | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + ovalbumin | - |
Escherichia coli | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + ovalbumin | - |
Homo sapiens | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + ovalbumin | - |
Rattus norvegicus | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + ovalbumin | - |
Triticum aestivum | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + ovalbumin | - |
Caenorhabditis elegans | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + ovalbumin | - |
Thermotoga maritima | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + protein L-isoaspartate | - |
Mus musculus | S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester | - |
? | |
S-adenosyl-L-methionine + protein L-isoaspartate | - |
Escherichia coli | S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester | - |
? | |
S-adenosyl-L-methionine + protein L-isoaspartate | - |
Homo sapiens | S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester | - |
? | |
S-adenosyl-L-methionine + protein L-isoaspartate | - |
Rattus norvegicus | S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester | - |
? | |
S-adenosyl-L-methionine + protein L-isoaspartate | - |
Triticum aestivum | S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester | - |
? | |
S-adenosyl-L-methionine + protein L-isoaspartate | - |
Methanothermobacter thermautotrophicus | S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester | - |
? | |
S-adenosyl-L-methionine + protein L-isoaspartate | - |
Caenorhabditis elegans | S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester | - |
? | |
S-adenosyl-L-methionine + protein L-isoaspartate | - |
Methanocaldococcus jannaschii | S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester | - |
? | |
S-adenosyl-L-methionine + protein L-isoaspartate | - |
Archaeoglobus fulgidus | S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester | - |
? | |
S-adenosyl-L-methionine + protein L-isoaspartate | - |
Thermotoga maritima | S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester | - |
? | |
S-adenosyl-L-methionine + protein L-isoaspartate | highly active protein repair enzyme | Thermotoga maritima | S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester | - |
? | |
S-adenosyl-L-methionine + VYP (isoD) HA | - |
Escherichia coli | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + VYP (isoD) HA | - |
Homo sapiens | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + VYP (isoD) HA | - |
Triticum aestivum | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + VYP (isoD) HA | - |
Caenorhabditis elegans | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + VYP (isoD) HA | - |
Thermotoga maritima | S-adenosyl-L-homocysteine + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Escherichia coli |
monomer | - |
Homo sapiens |
monomer | 1 * 34000, equilibrium centrifugation | Thermotoga maritima |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
- |
Thermotoga maritima |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 93 | - |
Thermotoga maritima |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
extremely heat stable, no loss of activity after 60 min | Thermotoga maritima |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Escherichia coli |
5.6 | - |
- |
Thermotoga maritima |
6 | 7 | - |
Rattus norvegicus |
7.5 | - |
- |
Rattus norvegicus |
7.5 | - |
- |
Triticum aestivum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8.5 | - |
Thermotoga maritima |