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Literature summary for 2.1.1.76 extracted from

  • Huang, T.S.; Anzellotti, D.; Dedaldechamp, F.; Ibrahim, R.K.
    Partial purification, kinetic analysis, and amino acid sequence information of a flavonol 3-O-methyltransferase from Serratula tinctoria (2004), Plant Physiol., 134, 1366-1376.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.76

Inhibitors

Inhibitors Comment Organism Structure
p-chloromercuribenzoate 1 mM, complete inhibition Serratula tinctoria

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.007
-
 myricetin 30°C Serratula tinctoria
0.012
-
 quercetin 30°C Serratula tinctoria
0.013
-
 kaempferol 30°C Serratula tinctoria
0.045
-
 S-adenosyl-L-methionine 30°C Serratula tinctoria

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
 1 * 31000, SDS-PAGE Serratula tinctoria
35000
-
 gel filtration Serratula tinctoria

Organism

Organism UniProt Comment Textmining
Serratula tinctoria
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Serratula tinctoria

Reaction

Reaction Comment Organism Reaction ID
S-adenosyl-L-methionine + 3,5,7,3',4'-pentahydroxyflavone = S-adenosyl-L-homocysteine + 3-methoxy-5,7,3',4'-tetrahydroxyflavone ordered sequential bi-bi mechanism Serratula tinctoria
a

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Serratula tinctoria
-
root
-
 Serratula tinctoria
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
13.8
-
-
 Serratula tinctoria

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + galangin 60% of the activity with quercetin Serratula tinctoria S-adenosyl-L-homocysteine + ?
-
 ?
S-adenosyl-L-methionine + isorhamnetin 88% of the activity with quercetin Serratula tinctoria S-adenosyl-L-homocysteine + ?
-
 ?
S-adenosyl-L-methionine + kaempferol 93% of the activity with quercetin Serratula tinctoria S-adenosyl-L-homocysteine + 3-O-methyl-kaempferol
-
 ?
S-adenosyl-L-methionine + myricetin 46% of the activity with quercetin Serratula tinctoria S-adenosyl-L-homocysteine + 3-O-methyl-myricetin
-
 ?
S-adenosyl-L-methionine + quercetin
-
 Serratula tinctoria S-adenosyl-L-homocysteine + 3-O-methyl-quercetin
-
 ?
S-adenosyl-L-methionine + tamarixetin 85% of the activity with quercetin Serratula tinctoria S-adenosyl-L-homocysteine + ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 31000, SDS-PAGE Serratula tinctoria

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
-
 Serratula tinctoria

pI Value

Organism Comment pI Value Maximum pI Value
Serratula tinctoria chromatofocusing
-
 6