Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Caulobacter vibrioides |
Crystallization (Comment) | Organism |
---|---|
in complex with double-stranded DNA containing the recognition sequence GANTC, sitting drop vapor diffusion method, using 0.1 M HEPES pH 7.8, with 10% (w/v) polyethylene glycol 8000 and 3% (w/v) polyethylene glycol 6000 | Caulobacter vibrioides |
Protein Variants | Comment | Organism |
---|---|---|
S315A/H317A/N330A/R350A | the mutant shows about 90% reduced methylation activity on double stranded DNA but no effect on S-adenosyl-L-methionione binding | Caulobacter vibrioides |
W332A | inactive | Caulobacter vibrioides |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + DNA adenine | Caulobacter vibrioides | the enzyme methylates the adenine of hemimethylated GANTC after replication | S-adenosyl-L-homocysteine + DNA 6-methyladenine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Caulobacter vibrioides | P0CAW2 | - |
- |
Purification (Comment) | Organism |
---|---|
HisTrap nickel affinity column chromatography | Caulobacter vibrioides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + DNA adenine | the enzyme methylates the adenine of hemimethylated GANTC after replication | Caulobacter vibrioides | S-adenosyl-L-homocysteine + DNA 6-methyladenine | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Caulobacter vibrioides |
Synonyms | Comment | Organism |
---|---|---|
CcrM | - |
Caulobacter vibrioides |
cell cycle-regulated DNA methyltransferase | - |
Caulobacter vibrioides |
DNA adenine methyltransferase | - |
Caulobacter vibrioides |