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Literature summary for 2.1.1.72 extracted from
- Functional analysis of an acid adaptive DNA adenine methyltransferase from Helicobacter pylori 26695 (2011), PLoS ONE, 6, e16810.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) pLysS cells | Helicobacter pylori |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C54G | the mutant has wild type activity | Helicobacter pylori |
| Y107G | inactive | Helicobacter pylori |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| S-adenosyl-L-methionine | methylation reaction is inhibited above 0.0015 mM of S-adenosyl-L-methionine | Helicobacter pylori |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00015 | - |
DNA adenine | at pH 5.5 and 37°C | Helicobacter pylori | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 3fold stimulation of activity at 1 mM | Helicobacter pylori | |
| Mg2+ | 3.5fold stimulation of activity at 1 mM | Helicobacter pylori | |
| Mn2+ | 5fold stimulation of activity at 1 mM | Helicobacter pylori | |
| additional information | Ca2+, Zn2+, and Ni2+ ions do not affect the methylation activity of the enzyme | Helicobacter pylori |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 70600 | - |
2 * 70600, the enzyme occurs as both monomer and dimer in solution, His-tagged enzyme, MALDI-TOF mass spectrometry | Helicobacter pylori |
| 72000 | - |
gel filtration, the enzyme occurs as both monomer and dimer in solution | Helicobacter pylori |
| 144000 | - |
gel filtration, the enzyme occurs as both monomer and dimer in solution | Helicobacter pylori |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Helicobacter pylori | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni2+-NTA column chromatography | Helicobacter pylori |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + DNA adenine | duplex 2 DNA, 5'-GCAG-3' is the recognition site for the enzyme | Helicobacter pylori | S-adenosyl-L-homocysteine + DNA 6-methyladenine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 70600, the enzyme occurs as both monomer and dimer in solution, His-tagged enzyme, MALDI-TOF mass spectrometry | Helicobacter pylori |
| monomer | 1 * 72000, the enzyme occurs as both monomer and dimer in solution, His-tagged enzyme, gel filtration | Helicobacter pylori |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HP0593 DNA-(N6-adenine)-methyltransferase | - |
Helicobacter pylori |
| HP0593 MTase | - |
Helicobacter pylori |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Helicobacter pylori |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Helicobacter pylori | isoelectric focusing | - |
5.8 |
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