Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE3) pLysS cells | Helicobacter pylori |
Protein Variants | Comment | Organism |
---|---|---|
F195S | the mutant is not able to bind to the S-adenosyl-L-methionine as effectively as the wild type enzyme and is catalytically inactive | Helicobacter pylori |
Y32L | the mutant binds to S-adenosyl-L-methionine as efficiently as wild type enzyme but is catalytically inactive | Helicobacter pylori |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0199 | - |
DNA adenine | in 10 mM Tris/HCl, pH 8.0, 5 mM 2-mercaptoethanol, at 37°C | Helicobacter pylori |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
gel filtration | Helicobacter pylori |
32000 | - |
1 * 32000, His-tagged enzyme, SDS-PAGE | Helicobacter pylori |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Helicobacter pylori | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Helicobacter pylori |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme recognizes and methylates GAGG (100% activity, Km 0.00522 mM), GGAG (about 48% activity, Km 0.017 mM) and GAAG (about 30% activity, Km 0.013 mM), but does not methylate GTGG, GmAmAG or GAGA | Helicobacter pylori | ? | - |
? | |
S-adenosyl-L-methionine + DNA adenine | HP0050 methyltransferase methylates one adenine at a time in the sequence 5'-GAAG-3' | Helicobacter pylori | S-adenosyl-L-homocysteine + DNA 6-methyladenine | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 32000, His-tagged enzyme, SDS-PAGE | Helicobacter pylori |
Synonyms | Comment | Organism |
---|---|---|
Dam | - |
Helicobacter pylori |
HP0050 methyltransferase | - |
Helicobacter pylori |
N6 adenine methyltransferase | - |
Helicobacter pylori |
N6 adenine MTase | - |
Helicobacter pylori |
General Information | Comment | Organism |
---|---|---|
malfunction | inactivation of the enzyme results in poor growth | Helicobacter pylori |