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Literature summary for 2.1.1.72 extracted from

  • Kumar, R.; Mukhopadhyay, A.K.; Rao, D.N.
    Characterization of an N6 adenine methyltransferase from Helicobacter pylori strain 26695 which methylates adjacent adenines on the same strand (2010), FEBS J., 277, 1666-1683.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) pLysS cells Helicobacter pylori

Protein Variants

Protein Variants Comment Organism
F195S the mutant is not able to bind to the S-adenosyl-L-methionine as effectively as the wild type enzyme and is catalytically inactive Helicobacter pylori
Y32L the mutant binds to S-adenosyl-L-methionine as efficiently as wild type enzyme but is catalytically inactive Helicobacter pylori

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0199
-
DNA adenine in 10 mM Tris/HCl, pH 8.0, 5 mM 2-mercaptoethanol, at 37°C Helicobacter pylori

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
gel filtration Helicobacter pylori
32000
-
1 * 32000, His-tagged enzyme, SDS-PAGE Helicobacter pylori

Organism

Organism UniProt Comment Textmining
Helicobacter pylori
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Helicobacter pylori

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme recognizes and methylates GAGG (100% activity, Km 0.00522 mM), GGAG (about 48% activity, Km 0.017 mM) and GAAG (about 30% activity, Km 0.013 mM), but does not methylate GTGG, GmAmAG or GAGA Helicobacter pylori ?
-
?
S-adenosyl-L-methionine + DNA adenine HP0050 methyltransferase methylates one adenine at a time in the sequence 5'-GAAG-3' Helicobacter pylori S-adenosyl-L-homocysteine + DNA 6-methyladenine
-
?

Subunits

Subunits Comment Organism
monomer 1 * 32000, His-tagged enzyme, SDS-PAGE Helicobacter pylori

Synonyms

Synonyms Comment Organism
Dam
-
Helicobacter pylori
HP0050 methyltransferase
-
Helicobacter pylori
N6 adenine methyltransferase
-
Helicobacter pylori
N6 adenine MTase
-
Helicobacter pylori

General Information

General Information Comment Organism
malfunction inactivation of the enzyme results in poor growth Helicobacter pylori