Cloned (Comment) | Organism |
---|---|
mutant enzymes are expressed in Escherichia coli HMS174(DE3) cells | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
R124R/P134A | the mutant shows an increase of enzyme activity at GAAC sites | Escherichia coli |
R124S/P134A | the mutant shows an increase of enzyme activity at GATG sites | Escherichia coli |
R124S/P134S | the mutant shows an increase of enzyme activity at GATT sites | Escherichia coli |
R124S/P134S/K139E/F159L/K241E | the mutant shows a more than 20fold preference for methylation at GATT, overall corresponding to a 1600fold change in specificity, the mutant is virtually inactive at GATC sites | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Escherichia coli | the wild type Dam shows no detectable activity at GATT sites | ? | - |
? | |
S-adenosyl-L-methionine + DNA adenine | Escherichia coli | the wild type enzyme shows target specificity for GATC sites | S-adenosyl-L-homocysteine + DNA 6-methyladenine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the wild type Dam shows no detectable activity at GATT sites | Escherichia coli | ? | - |
? | |
S-adenosyl-L-methionine + DNA adenine | the wild type enzyme shows target specificity for GATC sites | Escherichia coli | S-adenosyl-L-homocysteine + DNA 6-methyladenine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
adenine-N6 DNA methyltransferase | - |
Escherichia coli |
Dam | - |
Escherichia coli |
DNA-(adenine-N6)-methyltransferase | - |
Escherichia coli |