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Literature summary for 2.1.1.72 extracted from
- Competitive Lrp and Dam assembly at the pap regulatory region: implications for mechanisms of epigenetic regulation (2008), J. Mol. Biol., 383, 92-105.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | leucine-responsive regulatory protein specifically inhibits Dam methylation of GATC proximal to the pilus genes when pre-assembled onto a pap half-site substrate and when competing kinetically with Dam for access to this site, leucine-responsive regulatory protein does not efficiently inhibit Dam methylation of non-regulatory GATC sites in plasmid DNA, leucine-responsive regulatory protein inhibits Dam methylation of GATC proximal to the pilus genes in a pap substrate where GATC distal to the pilus genes is hemimethylated | Escherichia coli |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + DNA adenine | Escherichia coli | Dam methylates a GATC recognition site | S-adenosyl-L-homocysteine + DNA 6-methyladenine | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + DNA adenine | Dam methylates a GATC recognition site | Escherichia coli | S-adenosyl-L-homocysteine + DNA 6-methyladenine | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Dam | - |
Escherichia coli |
| DNA adenine methyltransferase | - |
Escherichia coli |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | Dam is a key regulator of the pap operon which codes for the pilus proteins necessary for uropathogenic Escherichia coli cellular adhesion. Lrp, in the presence and in the absence of PapI and nonspecific DNA, specifically protects pap regulatory GATC sites from Dam methylation when allowed to compete with Dam for assembly on unmethylated and hemimethylated pap DNA. Only at low Lrp concentrations will Dam compete effectively for binding and methylation of the proximal GATC site, leading to a phase switch resulting in the expression of pili | Escherichia coli |
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