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Literature summary for 2.1.1.72 extracted from

  • Coffin, S.R.; Reich, N.O.
    Escherichia coli DNA adenine methyltransferase: intrasite processivity and substrate-induced dimerization and activation (2009), Biochemistry, 48, 7399-7410.
    View publication on PubMed
Search for more literature for 2.1.1.72

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli XL2-Blue cells Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Escherichia coli the preferred substrate consists of the annealed product of oligonucleotides 5'-CATTTACTTGATCCGGTATGC-3' and 5'-GCATACCGGATCAAGTAAATG-3', while the nonpreferred substrate consists of the annealed product of oligonucleotides 5'-CATTTAGACGATCTTTTATGC-3' and 5'-GCATAAAAGATCGTCTAAATG-3' ?
-
 ?
S-adenosyl-L-methionine + DNA adenine Escherichia coli Dam belongs to the alpha-class of adenine methyltransferases and transfers a methyl group to the N-6 position of the adenine in the DNA sequence 5'-GATC-3' S-adenosyl-L-homocysteine + DNA 6-methyladenine
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
phosphocellulose column chromatography and Blue Sepharose column chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the preferred substrate consists of the annealed product of oligonucleotides 5'-CATTTACTTGATCCGGTATGC-3' and 5'-GCATACCGGATCAAGTAAATG-3', while the nonpreferred substrate consists of the annealed product of oligonucleotides 5'-CATTTAGACGATCTTTTATGC-3' and 5'-GCATAAAAGATCGTCTAAATG-3' Escherichia coli ?
-
 ?
S-adenosyl-L-methionine + DNA adenine Dam belongs to the alpha-class of adenine methyltransferases and transfers a methyl group to the N-6 position of the adenine in the DNA sequence 5'-GATC-3' Escherichia coli S-adenosyl-L-homocysteine + DNA 6-methyladenine
-
 ?

Subunits

Subunits Comment Organism
dimer Dam dimerizes on short, synthetic DNA, resulting in enhanced catalysis, however, dimerization is not observed on large genomic DNA Escherichia coli

Synonyms

Synonyms Comment Organism
Dam
-
 Escherichia coli
DNA adenine methyltransferase
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.003
-
 DNA adenine in 100 mM Tris (pH 8.0), 1 mM EDTA, 1 mM dithiothreitol, and 0.2 mg/ml bovine serum albumin, at 22°C Escherichia coli

General Information

General Information Comment Organism
physiological function DNA adenine methyltransferase is essential for proper DNA replication timing, gene regulation, and mismatch repair Escherichia coli