Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain ER2683 | Methylophilus methylotrophus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
105100 | - |
calculated from amino acid sequence | Methylophilus methylotrophus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Methylophilus methylotrophus | MmeI does not produce any detectable N4-cytosine methylation | ? | - |
? | |
S-adenosyl-L-methionine + DNA adenine | Methylophilus methylotrophus | MmeI modifies only the adenine in the top strand, 5-TCCRAC-3, MmeI endonuclease activity is blocked by this top strand adenine methylation and is unaffected by methylation of the adenine in the complementary strand, 5-GTYGGA-3' | S-adenosyl-L-homocysteine + DNA 6-methyladenine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylophilus methylotrophus | B2MU09 | - |
- |
Purification (Comment) | Organism |
---|---|
heparin Hyper D column chromatography, Source 15Q column chromatography, heparin TSK column chromatography, Source 15 S column chromatography, Superdex 75 gel filtration, and ceramic HTP column chromatography | Methylophilus methylotrophus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | MmeI does not produce any detectable N4-cytosine methylation | Methylophilus methylotrophus | ? | - |
? | |
S-adenosyl-L-methionine + DNA adenine | MmeI modifies only the adenine in the top strand, 5-TCCRAC-3, MmeI endonuclease activity is blocked by this top strand adenine methylation and is unaffected by methylation of the adenine in the complementary strand, 5-GTYGGA-3' | Methylophilus methylotrophus | S-adenosyl-L-homocysteine + DNA 6-methyladenine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MmeI | has both endonuclease and DNA methyltransferase activities | Methylophilus methylotrophus |
General Information | Comment | Organism |
---|---|---|
physiological function | MmeI DNA methylation prevents cleavage by MmeI | Methylophilus methylotrophus |