Literature summary for 2.1.1.72 extracted from

Faelker, S.; Schmidt, M.A.; Heusipp, G.
DNA methylation in Yersinia enterocolitica: role of the DNA adenine methyltransferase in mismatch repair and regulation of virulence factors (2005), Microbiology, 151, 2291-2299.

Application

Application	Comment	Organism
medicine	Dam is essential for viability and mediates virulenc	Yersinia enterocolitica

Cloned(Commentary)

Cloned (Comment)	Organism
ligated into KpnI/XbaI-digested pBluescriptIIKS+, Dam overproduction in Yersinia enterocolitica, which results in an increased frequency of spontaneous mutation and marginally resistance to 2-aminopurine, subcloned into pVLT33 under the control of an inducible Ptac promoter and introduced into the dam mutant strain Escherichia coli GM2163, can complement it, overproduction in CHO cells, lleads to increased invasion of Yersinia enterocolitica	Yersinia enterocolitica
overproduction in Yersinia enterocolitica, increased spontaneous resistance to chloramphenicol 21.2fold and to streptomycin 39.4fold, decrease in resistance to 2-aminopurine, expression in Yersinia enterocolitica mutant strain GHY121, can complement it	Escherichia coli

Cloned (Comment)

Organism

ligated into KpnI/XbaI-digested pBluescriptIIKS+, Dam overproduction in Yersinia enterocolitica, which results in an increased frequency of spontaneous mutation and marginally resistance to 2-aminopurine, subcloned into pVLT33 under the control of an inducible Ptac promoter and introduced into the dam mutant strain Escherichia coli GM2163, can complement it, overproduction in CHO cells, lleads to increased invasion of Yersinia enterocolitica

Yersinia enterocolitica

overproduction in Yersinia enterocolitica, increased spontaneous resistance to chloramphenicol 21.2fold and to streptomycin 39.4fold, decrease in resistance to 2-aminopurine, expression in Yersinia enterocolitica mutant strain GHY121, can complement it

Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
additional information	complementation of the Escherichia coli dam mutant strain GM2163 with the Yersinia enterocolitica dam gene	Escherichia coli
additional information	dam mutant, a CamS SmR SpR exconjugant can not be obtained	Yersinia enterocolitica

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
31200	-	sequence analysis	Yersinia enterocolitica

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	strains GHE131, GHE134, GHE135 and GM2163	-
Yersinia enterocolitica	-	strains GHY15, GHY121, GHY125, GHY128, GHY129, GHY130, GHY137, GHY138, GHY139, GHY140, GHY141, GHY142, GHY143, GHY144, GHY145, GHY146, GHY147, GHY148, GHY150, GHY151, GHY157, GHY158 and GHY226	-

Synonyms

Synonyms	Comment	Organism
Dam	-	Escherichia coli
Dam	-	Yersinia enterocolitica
DNA adenine methyltransferase	-	Escherichia coli
DNA adenine methyltransferase	-	Yersinia enterocolitica