Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme in binary complex with S-adenosyl-L-homocysteine or in ternary complex with a synthetic 12 bp DNA duplex and S-adenosyl-L-homocysteine, hanging drop vapour diffusion method, 16°C, reservoir solution contains 2.4 M ammonium sulfate and 100 mM MES, pH 6.0, for the binary complex, and contains 20-25% w/v PEG 8000, 100 mM HEPES, pH 7.5, and 10 mM ammonium sulfate for the ternary complex, X-ray diffraction structure determination and analysis at 2.3-3.5 A resolution, modeling | Tequatrovirus T4 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
about, recombinant enzyme, gel filtration | Tequatrovirus T4 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + DNA adenine | Tequatrovirus T4 | methylation of DNA-adenine at certain GATC sites plays a pivotal role in bacterial and phage gene expression as well as in bacterial virulence | S-adenosyl-L-homocysteine + DNA 6-methylaminopurine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tequatrovirus T4 | P04392 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme by several chromatographic steps | Tequatrovirus T4 |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
S-adenosyl-L-methionine + adenine in DNA = S-adenosyl-L-homocysteine + N6-methyladenine in DNA | active site structure, enzyme-methyl donor product interactions, substrate binding mechanism and conformational changes, overview | Tequatrovirus T4 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme binds also nonspecific to DNA, linear diffusion along the DNA, overview | Tequatrovirus T4 | ? | - |
? | |
S-adenosyl-L-methionine + DNA adenine | methylation of DNA-adenine at certain GATC sites plays a pivotal role in bacterial and phage gene expression as well as in bacterial virulence | Tequatrovirus T4 | S-adenosyl-L-homocysteine + DNA 6-methylaminopurine | - |
? | |
S-adenosyl-L-methionine + DNA adenine | specific methylation of target sequence GATC | Tequatrovirus T4 | S-adenosyl-L-homocysteine + DNA 6-methylaminopurine | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the enzyme contains 2 domains: a seven-stranded catalytic domain harboring the binding sites for S-adenosyl-L-homocysteine, and a DNA binding domain of a five-helix bundle and a beta-hairpin | Tequatrovirus T4 |
Synonyms | Comment | Organism |
---|---|---|
Dam MTase | - |
Tequatrovirus T4 |
DNA adenine methyltransferase | - |
Tequatrovirus T4 |
MTase | - |
Tequatrovirus T4 |
T4Dam | - |
Tequatrovirus T4 |