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Literature summary for 2.1.1.72 extracted from

  • Yang, Z.; Horton, J.R.; Zhou, L.; Zhang, X.J.; Dong, A.; Zhang, X.; Schlagman, S.L.; Kossykh, V.; Hattman, S.; Cheng, X.
    Structure of the bacteriophage T4 DNA adenine methyltransferase (2003), Nat. Struct. Biol., 10, 849-855.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant enzyme in binary complex with S-adenosyl-L-homocysteine or in ternary complex with a synthetic 12 bp DNA duplex and S-adenosyl-L-homocysteine, hanging drop vapour diffusion method, 16°C, reservoir solution contains 2.4 M ammonium sulfate and 100 mM MES, pH 6.0, for the binary complex, and contains 20-25% w/v PEG 8000, 100 mM HEPES, pH 7.5, and 10 mM ammonium sulfate for the ternary complex, X-ray diffraction structure determination and analysis at 2.3-3.5 A resolution, modeling Tequatrovirus T4

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
about, recombinant enzyme, gel filtration Tequatrovirus T4

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + DNA adenine Tequatrovirus T4 methylation of DNA-adenine at certain GATC sites plays a pivotal role in bacterial and phage gene expression as well as in bacterial virulence S-adenosyl-L-homocysteine + DNA 6-methylaminopurine
-
?

Organism

Organism UniProt Comment Textmining
Tequatrovirus T4 P04392
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme by several chromatographic steps Tequatrovirus T4

Reaction

Reaction Comment Organism Reaction ID
S-adenosyl-L-methionine + adenine in DNA = S-adenosyl-L-homocysteine + N6-methyladenine in DNA active site structure, enzyme-methyl donor product interactions, substrate binding mechanism and conformational changes, overview Tequatrovirus T4

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme binds also nonspecific to DNA, linear diffusion along the DNA, overview Tequatrovirus T4 ?
-
?
S-adenosyl-L-methionine + DNA adenine methylation of DNA-adenine at certain GATC sites plays a pivotal role in bacterial and phage gene expression as well as in bacterial virulence Tequatrovirus T4 S-adenosyl-L-homocysteine + DNA 6-methylaminopurine
-
?
S-adenosyl-L-methionine + DNA adenine specific methylation of target sequence GATC Tequatrovirus T4 S-adenosyl-L-homocysteine + DNA 6-methylaminopurine
-
?

Subunits

Subunits Comment Organism
More the enzyme contains 2 domains: a seven-stranded catalytic domain harboring the binding sites for S-adenosyl-L-homocysteine, and a DNA binding domain of a five-helix bundle and a beta-hairpin Tequatrovirus T4

Synonyms

Synonyms Comment Organism
Dam MTase
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Tequatrovirus T4
DNA adenine methyltransferase
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Tequatrovirus T4
MTase
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Tequatrovirus T4
T4Dam
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Tequatrovirus T4