Inhibitors | Comment | Organism | Structure |
---|---|---|---|
fully methylated sites 5'-GMTC/5'-GMTC DNA | the inhibition potential of fully methylated sites 5'-GMTC/5'-GMTC is much lower for a long DNA molecule compared to short single-site duplxes, enzyme does not dissociate from the DNA but continues one-sited methylation with linear diffusion, after which it rapidly reorientates itself and reconstitutes | Tequatrovirus T4 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | detailed steady-state and pre-steady-state kinetics for different methylation sites | Tequatrovirus T4 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Tequatrovirus T4 | S-adenosyl-L-methionine plays a crucial role in reorientation of the enzyme in DNA with mutationally altered Ade residues to 2-aminopurine, overview | ? | - |
? | |
S-adenosyl-L-methionine + DNA adenine | Tequatrovirus T4 | - |
S-adenosyl-L-homocysteine + DNA 6-methylaminopurine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tequatrovirus T4 | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
S-adenosyl-L-methionine + adenine in DNA = S-adenosyl-L-homocysteine + N6-methyladenine in DNA | ordered bi bi mechanism during progressive methylation process | Tequatrovirus T4 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | S-adenosyl-L-methionine plays a crucial role in reorientation of the enzyme in DNA with mutationally altered Ade residues to 2-aminopurine, overview | Tequatrovirus T4 | ? | - |
? | |
S-adenosyl-L-methionine + DNA adenine | - |
Tequatrovirus T4 | S-adenosyl-L-homocysteine + DNA 6-methylaminopurine | - |
? | |
S-adenosyl-L-methionine + DNA adenine | specific methylation of oligonucleotide duplexes containing one or two target sequence GATC sites | Tequatrovirus T4 | S-adenosyl-L-homocysteine + DNA 6-methylaminopurine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DNA-(N6-adenine)-methyltransferase | - |
Tequatrovirus T4 |
N6-Ade MTase | - |
Tequatrovirus T4 |
T4 Dam | - |
Tequatrovirus T4 |
T4 Dam DNA-(N6-adenine)-methyltransferase | - |
Tequatrovirus T4 |
T4 Dam MTase | - |
Tequatrovirus T4 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Tequatrovirus T4 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Tequatrovirus T4 | |
0.015 | - |
DNA adenine | 20mer duplex, unligated, single-site, pH 8.0, 25°C | Tequatrovirus T4 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Tequatrovirus T4 |