Cloned (Comment) | Organism |
---|---|
expression of the apo-enzyme mutant L72P as His-tagged enzyme | Cereibacter sphaeroides |
Crystallization (Comment) | Organism |
---|---|
enzyme complexed with substrate S-adenosyl-L-methionine, product S-adenosyl-L-homocysteine, or inhibitor sinefungin, and L72P mutant apo-enzyme, 2.0 mg/ml protein in crystallization buffer containing 100 mM HEPES, pH 7.4, 1.5 M LiSO4, and 1-20 mM of the ligand compounds, X-ray diffraction structure determination and analysis at 2.3 A resolution | Cereibacter sphaeroides |
Protein Variants | Comment | Organism |
---|---|---|
L72P | site-directed mutagenesis, altered secondary structure, the active site is pushed away from the ligand binding site, especially by altered position of Trp84 | Cereibacter sphaeroides |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
sinefungin | binding structure | Cereibacter sphaeroides |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + DNA adenine | Cereibacter sphaeroides | - |
S-adenosyl-L-homocysteine + DNA 6-methylaminopurine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cereibacter sphaeroides | P14751 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + DNA adenine | - |
Cereibacter sphaeroides | S-adenosyl-L-homocysteine + DNA 6-methylaminopurine | - |
? | |
S-adenosyl-L-methionine + DNA adenine | substrate and product binding site structures | Cereibacter sphaeroides | S-adenosyl-L-homocysteine + DNA 6-methylaminopurine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
M-RsrI | - |
Cereibacter sphaeroides |
N6_N4_MTase | - |
Cereibacter sphaeroides |
RsrI N6-adenine DNA methyltransferase | - |
Cereibacter sphaeroides |