Cloned (Comment) | Organism |
---|---|
cloning of T2 dam gene into expression vector pJW2, creation of T2-T4 hybrid genes in expression vector pJW2 | Escherichia phage T2 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme is inhibited at ionic strength greater than 0.2 M | Escherichia phage T2 | |
additional information | - |
Tequatrovirus T4 | |
S-adenosyl-L-homocysteine | - |
Escherichia phage T2 | |
S-adenosyl-L-homocysteine | - |
Tequatrovirus T4 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000000011 | - |
adenine in T4 gt- dam- DNA | nonglucosylated unmethylated T4 gt- dam- DNA | Tequatrovirus T4 | |
0.0000000011 | - |
adenine in T4 gt- dam- DNA | nonglucosylated unmethylated T4 gt- dam- DNA | Escherichia phage T2 | |
0.0001 | - |
S-adenosyl-L-methionine | - |
Tequatrovirus T4 | |
0.0001 | - |
S-adenosyl-L-methionine | - |
Escherichia phage T2 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia phage T2 | - |
- |
- |
Tequatrovirus T4 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia phage T2 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Tequatrovirus T4 |
additional information | - |
- |
Escherichia phage T2 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + adenine in T4 gt- dam- DNA | - |
Tequatrovirus T4 | S-adenosyl-L-homocysteine + N6-methyladenine in T4 gt- dam- DNA | - |
? | |
S-adenosyl-L-methionine + adenine in T4 gt- dam- DNA | - |
Escherichia phage T2 | S-adenosyl-L-homocysteine + N6-methyladenine in T4 gt- dam- DNA | - |
? | |
S-adenosyl-L-methionine + DNA adenine | nonglucosylated, hydroxymethylcytosine-containing T2gt- virion DNA has a higher level of methylation than T4gt virion DNA does | Tequatrovirus T4 | S-adenosyl-L-homocysteine + DNA 6-methylaminopurine | - |
? | |
S-adenosyl-L-methionine + DNA adenine | nonglucosylated, hydroxymethylcytosine-containing T2gt- virion DNA has a higher level of methylation than T4gt virion DNA does | Escherichia phage T2 | S-adenosyl-L-homocysteine + DNA 6-methylaminopurine | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Tequatrovirus T4 | |
additional information | - |
additional information | residues at position 20 and 26 are responsible for the different turnover-number values of the two MTases bor both canonical and noncanonical sites | Escherichia phage T2 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8.5 | - |
Escherichia phage T2 |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0024 | - |
S-adenosyl-L-homocysteine | - |
Tequatrovirus T4 | |
0.0024 | - |
S-adenosyl-L-homocysteine | - |
Escherichia phage T2 |