BRENDA - Enzyme Database
show all sequences of 2.1.1.71

Conversion of phosphatidylethanolamine to phosphatidylcholine in rat liver. Partial purification and characterization of the enzymatic activities

Schneider, W.J.; Vance, D.E.; J. Biol. Chem. 254, 3886-3891 (1979)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
phosphatidylcholine
inhibits the incorporation of [3H]-methyl groups from S-adenosyl-L-methionine into phosphatidyl-N-monomethylethanolamine and phosphatidyl-N,N-dimethylethanolamine
Rattus norvegicus
S-adenosyl-L-homocysteine
competitive inhibition
Rattus norvegicus
Triton X-100
-
Rattus norvegicus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.016
-
S-adenosyl-L-methionine
substrate phosphatidyl-N,N-dimethylethanolamine
Rattus norvegicus
0.022
-
S-adenosyl-L-methionine
-
Rattus norvegicus
0.08
-
phosphatidyl-N-monomethylethanolamine
-
Rattus norvegicus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Rattus norvegicus
5737
-
microsome
-
Rattus norvegicus
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rattus norvegicus
-
; rat
-
Rattus norvegicus Wistar
-
-
-
Purification (Commentary)
Commentary
Organism
partial
Rattus norvegicus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
hepatocyte
-
Rattus norvegicus
-
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.79
-
-
Rattus norvegicus
Storage Stability
Storage Stability
Organism
-70°C, not stable to freezing
Rattus norvegicus
2°C, loses 30% of its activity within 12 h
Rattus norvegicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + phosphatidyl-N,N-dimethylethanolamine
-
485554
Rattus norvegicus
S-adenosyl-L-homocysteine + phosphatidylcholine
-
-
-
?
S-adenosyl-L-methionine + phosphatidyl-N,N-dimethylethanolamine
-
485554
Rattus norvegicus Wistar
S-adenosyl-L-homocysteine + phosphatidylcholine
-
-
-
?
S-adenosyl-L-methionine + phosphatidyl-N-monomethylethanolamine
-
485554
Rattus norvegicus
S-adenosyl-L-homocysteine + phosphatidyl-N-dimethylethanolamine
-
-
-
?
S-adenosyl-L-methionine + phosphatidyl-N-monomethylethanolamine
-
485554
Rattus norvegicus Wistar
S-adenosyl-L-homocysteine + phosphatidyl-N-dimethylethanolamine
-
-
-
?
S-adenosyl-L-methionine + phosphatidyl-N-monomethylethanolamine
-
485554
Rattus norvegicus
S-adenosyl-L-homocysteine + phosphatidylcholine
-
-
-
?
S-adenosyl-L-methionine + phosphatidyl-N-monomethylethanolamine
-
485554
Rattus norvegicus Wistar
S-adenosyl-L-homocysteine + phosphatidylcholine
-
-
-
?
S-adenosyl-L-methionine + phosphatidylethanolamine
-
485554
Rattus norvegicus
S-adenosyl-L-homocysteine + phosphatidyl-N-monomethylethanolamine
-
-
-
?
S-adenosyl-L-methionine + phosphatidylethanolamine
-
485554
Rattus norvegicus Wistar
S-adenosyl-L-homocysteine + phosphatidyl-N-monomethylethanolamine
-
-
-
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.5
-
-
Rattus norvegicus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5.5
9.5
-
Rattus norvegicus
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.0049
-
S-adenosyl-L-homocysteine
-
Rattus norvegicus
0.0067
-
S-adenosyl-L-homocysteine
inhibition of the last step in the synthesis of phosphatidylcholine
Rattus norvegicus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
phosphatidylcholine
inhibits the incorporation of [3H]-methyl groups from S-adenosyl-L-methionine into phosphatidyl-N-monomethylethanolamine and phosphatidyl-N,N-dimethylethanolamine
Rattus norvegicus
S-adenosyl-L-homocysteine
competitive inhibition
Rattus norvegicus
Triton X-100
-
Rattus norvegicus
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.0049
-
S-adenosyl-L-homocysteine
-
Rattus norvegicus
0.0067
-
S-adenosyl-L-homocysteine
inhibition of the last step in the synthesis of phosphatidylcholine
Rattus norvegicus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.016
-
S-adenosyl-L-methionine
substrate phosphatidyl-N,N-dimethylethanolamine
Rattus norvegicus
0.022
-
S-adenosyl-L-methionine
-
Rattus norvegicus
0.08
-
phosphatidyl-N-monomethylethanolamine
-
Rattus norvegicus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Rattus norvegicus
5737
-
microsome
-
Rattus norvegicus
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Rattus norvegicus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
hepatocyte
-
Rattus norvegicus
-
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.79
-
-
Rattus norvegicus
Storage Stability (protein specific)
Storage Stability
Organism
-70°C, not stable to freezing
Rattus norvegicus
2°C, loses 30% of its activity within 12 h
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + phosphatidyl-N,N-dimethylethanolamine
-
485554
Rattus norvegicus
S-adenosyl-L-homocysteine + phosphatidylcholine
-
-
-
?
S-adenosyl-L-methionine + phosphatidyl-N,N-dimethylethanolamine
-
485554
Rattus norvegicus Wistar
S-adenosyl-L-homocysteine + phosphatidylcholine
-
-
-
?
S-adenosyl-L-methionine + phosphatidyl-N-monomethylethanolamine
-
485554
Rattus norvegicus
S-adenosyl-L-homocysteine + phosphatidyl-N-dimethylethanolamine
-
-
-
?
S-adenosyl-L-methionine + phosphatidyl-N-monomethylethanolamine
-
485554
Rattus norvegicus Wistar
S-adenosyl-L-homocysteine + phosphatidyl-N-dimethylethanolamine
-
-
-
?
S-adenosyl-L-methionine + phosphatidyl-N-monomethylethanolamine
-
485554
Rattus norvegicus
S-adenosyl-L-homocysteine + phosphatidylcholine
-
-
-
?
S-adenosyl-L-methionine + phosphatidyl-N-monomethylethanolamine
-
485554
Rattus norvegicus Wistar
S-adenosyl-L-homocysteine + phosphatidylcholine
-
-
-
?
S-adenosyl-L-methionine + phosphatidylethanolamine
-
485554
Rattus norvegicus
S-adenosyl-L-homocysteine + phosphatidyl-N-monomethylethanolamine
-
-
-
?
S-adenosyl-L-methionine + phosphatidylethanolamine
-
485554
Rattus norvegicus Wistar
S-adenosyl-L-homocysteine + phosphatidyl-N-monomethylethanolamine
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.5
-
-
Rattus norvegicus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5.5
9.5
-
Rattus norvegicus
Other publictions for EC 2.1.1.71
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
718778
Morita
Functional analysis of two iso ...
Homo sapiens
Biochem. J.
432
387-398
2010
-
-
1
-
-
-
-
-
1
-
2
-
-
1
-
1
-
-
-
-
1
-
1
-
1
-
-
-
1
-
-
-
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1
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1
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2
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1
-
-
-
1
-
1
-
1
-
-
-
1
-
-
-
2
2
2
2
-
-
704482
Keogh
Functional characterization of ...
Arabidopsis thaliana, Glycine max
J. Biol. Chem.
284
15439-15447
2009
-
-
2
-
-
-
-
-
2
-
-
-
-
2
-
-
-
-
-
-
-
-
6
-
-
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-
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2
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2
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-
-
6
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
485190
Guan
Activity of phosphatidylethano ...
Homo sapiens
Neurochem. Int.
34
41-47
1999
-
-
-
-
-
-
-
-
-
-
-
-
-
1
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-
-
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-
-
-
-
-
-
485557
Kanipes
The phospholipid methyltransfe ...
Bos taurus, Mus musculus, Rattus norvegicus, Saccharomyces cerevisiae, Schizosaccharomyces pombe
Biochim. Biophys. Acta
1348
134-141
1997
-
-
4
-
-
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2
-
1
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5
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2
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4
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2
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1
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2
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-
-
-
-
-
485559
Iliskovic
Adriamycin depresses in vivo a ...
Rattus norvegicus, Rattus norvegicus Sprague-Dawley
Mol. Cell. Biochem.
176
235-240
1997
-
1
-
-
-
-
1
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-
162
-
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1
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2
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1
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1
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1
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2
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-
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-
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-
-
-
-
-
-
-
-
-
485193
Roque
Phosphatidylethanolamine N-met ...
Bos taurus, Rattus norvegicus
Exp. Eye Res.
60
631-643
1995
-
-
-
-
-
-
-
2
5
1
-
-
-
2
-
-
1
-
-
3
-
-
4
-
-
-
-
-
3
1
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
2
5
1
-
-
-
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1
-
3
-
-
4
-
-
-
-
-
3
1
-
-
-
-
-
-
-
-
485166
Gaynor
Phosphatidylethanolamine methy ...
Saccharomyces cerevisiae
Biochim. Biophys. Acta
1045
156-163
1990
-
-
1
-
-
-
6
4
-
2
-
-
-
2
-
-
-
-
-
-
-
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2
-
1
-
1
-
1
1
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4
-
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1
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6
4
4
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2
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-
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2
-
1
-
1
-
1
1
-
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-
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-
-
-
-
485561
Tanaka
Purification and properties of ...
Mus musculus
Jpn. J. Med. Sci. Biol.
43
59-73
1990
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1
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1
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1
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485562
Pilarska
Properties and topology of enz ...
Oryctolagus cuniculus
Int. J. Biochem.
19
705-711
1987
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1
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1
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1
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485560
Sastry
Evidence for two methyltransfe ...
Bos taurus, Homo sapiens, Neurospora crassa, Rattus norvegicus
Arch. Biochem. Biophys.
211
762-773
1981
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-
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2
6
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4
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8
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4
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2
1
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2
6
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8
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4
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2
1
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-
-
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485554
Schneider
Conversion of phosphatidyletha ...
Rattus norvegicus, Rattus norvegicus Wistar
J. Biol. Chem.
254
3886-3891
1979
-
-
-
-
-
-
3
3
2
-
-
-
-
166
-
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1
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2
1
2
8
-
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1
1
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2
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3
2
3
2
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1
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2
1
2
8
-
-
-
-
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1
1
-
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-
-
-
-
-
-
485555
Hirata
Identification and properties ...
Bos taurus, Neurospora crassa, Rattus norvegicus
Proc. Natl. Acad. Sci. USA
75
1718-1721
1978
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1
4
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3
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