BRENDA - Enzyme Database
show all sequences of 2.1.1.71

Phosphatidylethanolamine N-methyltransferase activity in isolated rod outer segments from bovine retina

Roque, M.E.; Giusto, N.M.; Exp. Eye Res. 60, 631-643 (1995)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.037
-
S-adenosyl-L-methionine
pH 8.5
Bos taurus
0.038
-
S-adenosyl-L-methionine
pH 10.0
Bos taurus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Bos taurus
5829
-
membrane
synaptosomal plasma membrane
Rattus norvegicus
16020
-
microsome
-
Bos taurus
-
-
microsome
-
Rattus norvegicus
-
-
mitochondrion
-
Bos taurus
5739
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
requires magnesium ions for full activity
Bos taurus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bos taurus
-
bovine
-
Rattus norvegicus
-
rat
-
Purification (Commentary)
Commentary
Organism
-
Rattus norvegicus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
eye
-
Bos taurus
-
liver
-
Rattus norvegicus
-
retina
rod outer segments
Bos taurus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + phosphatidyl-N,N-dimethylethanolamine
-
485193
Rattus norvegicus
S-adenosyl-L-homocysteine + phosphatidylcholine
-
-
-
?
S-adenosyl-L-methionine + phosphatidyl-N,N-dimethylethanolamine
-
485193
Bos taurus
S-adenosyl-L-homocysteine + phosphatidylcholine
-
-
-
?
S-adenosyl-L-methionine + phosphatidylethanolamine
-
485193
Rattus norvegicus
S-adenosyl-L-homocysteine + phosphatidyl-N-monomethylethanolamine
-
-
-
?
S-adenosyl-L-methionine + phosphatidylethanolamine
-
485193
Bos taurus
S-adenosyl-L-homocysteine + phosphatidyl-N-monomethylethanolamine
-
-
-
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
low substrate concentrations about 0.01 M S-adenosyl-L-methionine
Bos taurus
10
-
high substrate concentrations about 0.2 M S-adenosyl-L-methionine
Bos taurus
10
-
-
Rattus norvegicus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7
11
-
Bos taurus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.037
-
S-adenosyl-L-methionine
pH 8.5
Bos taurus
0.038
-
S-adenosyl-L-methionine
pH 10.0
Bos taurus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Bos taurus
5829
-
membrane
synaptosomal plasma membrane
Rattus norvegicus
16020
-
microsome
-
Bos taurus
-
-
microsome
-
Rattus norvegicus
-
-
mitochondrion
-
Bos taurus
5739
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
requires magnesium ions for full activity
Bos taurus
Purification (Commentary) (protein specific)
Commentary
Organism
-
Rattus norvegicus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
eye
-
Bos taurus
-
liver
-
Rattus norvegicus
-
retina
rod outer segments
Bos taurus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + phosphatidyl-N,N-dimethylethanolamine
-
485193
Rattus norvegicus
S-adenosyl-L-homocysteine + phosphatidylcholine
-
-
-
?
S-adenosyl-L-methionine + phosphatidyl-N,N-dimethylethanolamine
-
485193
Bos taurus
S-adenosyl-L-homocysteine + phosphatidylcholine
-
-
-
?
S-adenosyl-L-methionine + phosphatidylethanolamine
-
485193
Rattus norvegicus
S-adenosyl-L-homocysteine + phosphatidyl-N-monomethylethanolamine
-
-
-
?
S-adenosyl-L-methionine + phosphatidylethanolamine
-
485193
Bos taurus
S-adenosyl-L-homocysteine + phosphatidyl-N-monomethylethanolamine
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
low substrate concentrations about 0.01 M S-adenosyl-L-methionine
Bos taurus
10
-
high substrate concentrations about 0.2 M S-adenosyl-L-methionine
Bos taurus
10
-
-
Rattus norvegicus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7
11
-
Bos taurus
Other publictions for EC 2.1.1.71
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
718778
Morita
Functional analysis of two iso ...
Homo sapiens
Biochem. J.
432
387-398
2010
-
-
1
-
-
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-
-
1
-
2
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-
1
-
1
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1
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1
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1
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-
1
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1
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1
-
2
-
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-
1
-
-
-
1
-
1
-
1
-
-
-
1
-
-
-
2
2
2
2
-
-
704482
Keogh
Functional characterization of ...
Arabidopsis thaliana, Glycine max
J. Biol. Chem.
284
15439-15447
2009
-
-
2
-
-
-
-
-
2
-
-
-
-
2
-
-
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6
-
-
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2
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2
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6
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
485190
Guan
Activity of phosphatidylethano ...
Homo sapiens
Neurochem. Int.
34
41-47
1999
-
-
-
-
-
-
-
-
-
-
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1
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-
485557
Kanipes
The phospholipid methyltransfe ...
Bos taurus, Mus musculus, Rattus norvegicus, Saccharomyces cerevisiae, Schizosaccharomyces pombe
Biochim. Biophys. Acta
1348
134-141
1997
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4
-
-
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2
-
1
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5
-
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2
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4
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2
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1
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2
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-
485559
Iliskovic
Adriamycin depresses in vivo a ...
Rattus norvegicus, Rattus norvegicus Sprague-Dawley
Mol. Cell. Biochem.
176
235-240
1997
-
1
-
-
-
-
1
-
-
-
-
-
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162
-
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1
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2
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1
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1
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1
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-
2
-
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-
-
-
485193
Roque
Phosphatidylethanolamine N-met ...
Bos taurus, Rattus norvegicus
Exp. Eye Res.
60
631-643
1995
-
-
-
-
-
-
-
2
5
1
-
-
-
2
-
-
1
-
-
3
-
-
4
-
-
-
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
5
1
-
-
-
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-
1
-
3
-
-
4
-
-
-
-
-
3
1
-
-
-
-
-
-
-
-
485166
Gaynor
Phosphatidylethanolamine methy ...
Saccharomyces cerevisiae
Biochim. Biophys. Acta
1045
156-163
1990
-
-
1
-
-
-
6
4
-
2
-
-
-
2
-
-
-
-
-
-
-
-
2
-
1
-
1
-
1
1
-
-
4
-
-
-
-
1
-
-
-
-
-
6
4
4
-
2
-
-
-
-
-
-
-
-
-
-
2
-
1
-
1
-
1
1
-
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-
-
-
-
-
-
485561
Tanaka
Purification and properties of ...
Mus musculus
Jpn. J. Med. Sci. Biol.
43
59-73
1990
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-
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1
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1
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1
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-
-
-
-
-
485562
Pilarska
Properties and topology of enz ...
Oryctolagus cuniculus
Int. J. Biochem.
19
705-711
1987
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-
-
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-
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1
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1
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1
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1
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1
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-
-
-
485560
Sastry
Evidence for two methyltransfe ...
Bos taurus, Homo sapiens, Neurospora crassa, Rattus norvegicus
Arch. Biochem. Biophys.
211
762-773
1981
-
-
-
-
-
-
-
2
6
-
-
-
-
4
-
-
-
-
-
8
-
-
4
-
-
-
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-
2
1
-
-
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-
-
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-
-
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-
-
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-
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2
6
-
-
-
-
-
-
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-
8
-
-
4
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
485554
Schneider
Conversion of phosphatidyletha ...
Rattus norvegicus, Rattus norvegicus Wistar
J. Biol. Chem.
254
3886-3891
1979
-
-
-
-
-
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3
3
2
-
-
-
-
166
-
-
1
-
-
2
1
2
8
-
-
-
-
-
1
1
-
-
2
-
-
-
-
-
-
-
-
-
-
3
2
3
2
-
-
-
-
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-
1
-
2
1
2
8
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
485555
Hirata
Identification and properties ...
Bos taurus, Neurospora crassa, Rattus norvegicus
Proc. Natl. Acad. Sci. USA
75
1718-1721
1978
-
-
-
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1
4
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3
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4
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5
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2
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1
4
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4
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5
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2
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