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Literature summary for 2.1.1.7 extracted from

  • Chen, X.; Wood, A.J.
    Purification and characterization of S-adenosyl-L-methionine:nicotinic acid-N-methyltransferase from leaves of Glycine max (2004), Biol. Plant., 48, 531-535.
No PubMed abstract available

General Stability

General Stability Organism
10 mM DTT and 20% glycerol stabilize the unstable enzyme during purification and storage Glycine max

Inhibitors

Inhibitors Comment Organism Structure
Hg2+ at 1 mM, reversible by 10 mM DTT Glycine max
parahydroxymercuric benzoate at 0.1 mM, reversible by 10 mM DTT Glycine max

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0125
-
nicotinate pH 7.0, 35°C Glycine max
0.031
-
S-adenosyl-L-methionine pH 7.0, 35°C Glycine max

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41500
-
2 * 41500, SDS-PAGE Glycine max
85000
-
ge filtration Glycine max

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + nicotinate Glycine max key enzyme in regulating the metabolic flux through the nicotinamide pathway S-adenosyl-L-homocysteine + N-methylnicotinate
-
?

Organism

Organism UniProt Comment Textmining
Glycine max
-
-
-

Purification (Commentary)

Purification (Comment) Organism
2650fold from leaves, to homogeneity, by ammonium sulfate fractionation, DEAE ion exchange chromatography, adenosine affinity chromatography, hydroxyl apatite chromatography, and gel filtration Glycine max

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Glycine max
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0031
-
purified enzyme Glycine max

Storage Stability

Storage Stability Organism
-20°C, purified enzyme, 20 mM Tris-HCl, pH 8.0, 20 mM DTT, 20% glycerol, stable for 14 days Glycine max
4°C, purified enzyme, 20 mM Tris-HCl, pH 8.0, 20 mM DTT, 20% glycerol, stable for 3 days Glycine max
4°C, purified enzyme, 20 mM Tris-HCl, pH 8.0, 20% glycerol, complete loss of activity within 24 h Glycine max

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + nicotinate key enzyme in regulating the metabolic flux through the nicotinamide pathway Glycine max S-adenosyl-L-homocysteine + N-methylnicotinate
-
?
S-adenosyl-L-methionine + nicotinate specific for nicotinic acid Glycine max S-adenosyl-L-homocysteine + N-methylnicotinate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 41500, SDS-PAGE Glycine max

Synonyms

Synonyms Comment Organism
NNMT
-
Glycine max
S-adenosyl-L-methionine:nicotinic acid-N-methyltransferase
-
Glycine max

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40 45
-
Glycine max

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 50 50% remaining activity at 50°C Glycine max

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Glycine max

pH Range

pH Minimum pH Maximum Comment Organism
4.3 8.8 half-maximal activity at pH 5.4 and pH 7.5 Glycine max