General Stability | Organism |
---|---|
10 mM DTT and 20% glycerol stabilize the unstable enzyme during purification and storage | Glycine max |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Hg2+ | at 1 mM, reversible by 10 mM DTT | Glycine max | |
parahydroxymercuric benzoate | at 0.1 mM, reversible by 10 mM DTT | Glycine max |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0125 | - |
nicotinate | pH 7.0, 35°C | Glycine max | |
0.031 | - |
S-adenosyl-L-methionine | pH 7.0, 35°C | Glycine max |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41500 | - |
2 * 41500, SDS-PAGE | Glycine max |
85000 | - |
ge filtration | Glycine max |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + nicotinate | Glycine max | key enzyme in regulating the metabolic flux through the nicotinamide pathway | S-adenosyl-L-homocysteine + N-methylnicotinate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | - |
- |
- |
Purification (Comment) | Organism |
---|---|
2650fold from leaves, to homogeneity, by ammonium sulfate fractionation, DEAE ion exchange chromatography, adenosine affinity chromatography, hydroxyl apatite chromatography, and gel filtration | Glycine max |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Glycine max | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0031 | - |
purified enzyme | Glycine max |
Storage Stability | Organism |
---|---|
-20°C, purified enzyme, 20 mM Tris-HCl, pH 8.0, 20 mM DTT, 20% glycerol, stable for 14 days | Glycine max |
4°C, purified enzyme, 20 mM Tris-HCl, pH 8.0, 20 mM DTT, 20% glycerol, stable for 3 days | Glycine max |
4°C, purified enzyme, 20 mM Tris-HCl, pH 8.0, 20% glycerol, complete loss of activity within 24 h | Glycine max |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + nicotinate | key enzyme in regulating the metabolic flux through the nicotinamide pathway | Glycine max | S-adenosyl-L-homocysteine + N-methylnicotinate | - |
? | |
S-adenosyl-L-methionine + nicotinate | specific for nicotinic acid | Glycine max | S-adenosyl-L-homocysteine + N-methylnicotinate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 41500, SDS-PAGE | Glycine max |
Synonyms | Comment | Organism |
---|---|---|
NNMT | - |
Glycine max |
S-adenosyl-L-methionine:nicotinic acid-N-methyltransferase | - |
Glycine max |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 45 | - |
Glycine max |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 50 | 50% remaining activity at 50°C | Glycine max |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Glycine max |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.3 | 8.8 | half-maximal activity at pH 5.4 and pH 7.5 | Glycine max |