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Literature summary for 2.1.1.69 extracted from

  • Hauffe, K.D.; Hahlbrock, K.; Scheel, D.
    Elicitor-stimulated furanocoumarin biosynthesis in cultured parsley cells: S-adenosyl-L-methionine:bergaptol and S-adenosyl-L-methionine:xanthotoxol O-methyltransferase (1986), Z. Naturforsch. C, 41, 228-239.
No PubMed abstract available
Search for more literature for 2.1.1.69

General Stability

General Stability Organism
2-mercaptoethanol stabilizes during purification and storage Petroselinum crispum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
 5-hydroxyxanthotoxin
-
 Petroselinum crispum
0.0031
-
 S-adenosyl-L-methionine reaction with bergaptol or 5-hydroxyxanthotoxin Petroselinum crispum
0.004
-
 bergaptol
-
 Petroselinum crispum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 2 * 36000, SDS-PAGE Petroselinum crispum
73000
-
 gel filtration Petroselinum crispum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + bergaptol Petroselinum crispum final methylation in the biosynthesis of the furanocoumarin bergapten ?
-
 ?

Organism

Organism UniProt Comment Textmining
Petroselinum crispum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Petroselinum crispum

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture from leaf petiols, cells activated by an elicitor from Phytophtora megasperma, no activity in untreated cells Petroselinum crispum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.041
-
-
 Petroselinum crispum

Storage Stability

Storage Stability Organism
-20 C, stable for 20 days in 50% v/v glycerol Petroselinum crispum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + 5,7-dihydroxycoumarin 6% of the activity with bergaptol Petroselinum crispum S-adenosyl-L-homocysteine + citropten
-
 ?
S-adenosyl-L-methionine + 5,8-dihydroxypsoralen 2.6% of the activity with bergaptol Petroselinum crispum S-adenosyl-L-homocysteine + ?
-
 ?
S-adenosyl-L-methionine + 5-hydroxyxanthotoxin best substrate Petroselinum crispum S-adenosyl-L-homocysteine + isopimpinellin
-
 ?
S-adenosyl-L-methionine + bergaptol
-
 Petroselinum crispum S-adenosyl-L-homocysteine + bergapten
-
 ?
S-adenosyl-L-methionine + bergaptol final methylation in the biosynthesis of the furanocoumarin bergapten Petroselinum crispum ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 36000, SDS-PAGE Petroselinum crispum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.5
-
 Petroselinum crispum

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9.3 pH 6.5: about 30% of maximal activity, pH 9.3: about 45% of maximal activity Petroselinum crispum

pH Stability

pH Stability pH Stability Maximum Comment Organism
5
-
 below: inactivation within 30 min Petroselinum crispum