General Stability | Organism |
---|---|
2-mercaptoethanol stabilizes during purification and storage | Petroselinum crispum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
5-hydroxyxanthotoxin | - |
Petroselinum crispum | |
0.0031 | - |
S-adenosyl-L-methionine | reaction with bergaptol or 5-hydroxyxanthotoxin | Petroselinum crispum | |
0.004 | - |
bergaptol | - |
Petroselinum crispum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
2 * 36000, SDS-PAGE | Petroselinum crispum |
73000 | - |
gel filtration | Petroselinum crispum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + bergaptol | Petroselinum crispum | final methylation in the biosynthesis of the furanocoumarin bergapten | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Petroselinum crispum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Petroselinum crispum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell suspension culture | from leaf petiols, cells activated by an elicitor from Phytophtora megasperma, no activity in untreated cells | Petroselinum crispum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.041 | - |
- |
Petroselinum crispum |
Storage Stability | Organism |
---|---|
-20 C, stable for 20 days in 50% v/v glycerol | Petroselinum crispum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 5,7-dihydroxycoumarin | 6% of the activity with bergaptol | Petroselinum crispum | S-adenosyl-L-homocysteine + citropten | - |
? | |
S-adenosyl-L-methionine + 5,8-dihydroxypsoralen | 2.6% of the activity with bergaptol | Petroselinum crispum | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + 5-hydroxyxanthotoxin | best substrate | Petroselinum crispum | S-adenosyl-L-homocysteine + isopimpinellin | - |
? | |
S-adenosyl-L-methionine + bergaptol | - |
Petroselinum crispum | S-adenosyl-L-homocysteine + bergapten | - |
? | |
S-adenosyl-L-methionine + bergaptol | final methylation in the biosynthesis of the furanocoumarin bergapten | Petroselinum crispum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 36000, SDS-PAGE | Petroselinum crispum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.5 | - |
Petroselinum crispum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9.3 | pH 6.5: about 30% of maximal activity, pH 9.3: about 45% of maximal activity | Petroselinum crispum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | - |
below: inactivation within 30 min | Petroselinum crispum |