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Literature summary for 2.1.1.68 extracted from

  • Moinuddin, S.G.; Jourdes, M.; Laskar, D.D.; Ki, C.; Cardenas, C.L.; Kim, K.W.; Zhang, D.; Davin, L.B.; Lewis, N.G.
    Insights into lignin primary structure and deconstruction from Arabidopsis thaliana COMT (caffeic acid O-methyl transferase) mutant Atomt1 (2010), Org. Biomol. Chem., 8, 3928-3946.
    View publication on PubMed
Search for more literature for 2.1.1.68

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0179
-
 5-hydroxyconiferyl aldehyde pH 7.5, temperature not specified in the publication Arabidopsis thaliana
0.0197
-
 caffeoyl aldehyde pH 7.5, temperature not specified in the publication Arabidopsis thaliana
0.0237
-
 quercetin pH 7.5, temperature not specified in the publication Arabidopsis thaliana
0.0242
-
 caffeic acid pH 7.5, temperature not specified in the publication Arabidopsis thaliana
0.0316
-
 5-hydroxyconiferyl alcohol pH 7.5, temperature not specified in the publication Arabidopsis thaliana
0.032
-
 5-hydroxyferulic acid pH 7.5, temperature not specified in the publication Arabidopsis thaliana
0.0515
-
 caffeoyl alcohol pH 7.5, temperature not specified in the publication Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
 17 genes are annotated as putative COMTs in Arabidopsis
-

Purification (Commentary)

Purification (Comment) Organism
using affinity chromatography Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + 5-hydroxyconiferyl alcohol
-
 Arabidopsis thaliana ?
-
 ?
S-adenosyl-L-methionine + 5-hydroxyconiferyl aldehyde
-
 Arabidopsis thaliana ?
-
 ?
S-adenosyl-L-methionine + 5-hydroxyferulic acid
-
 Arabidopsis thaliana S-adenosyl-L-homocysteine + sinapic acid
-
 ?
S-adenosyl-L-methionine + caffeic acid
-
 Arabidopsis thaliana ?
-
 ?
S-adenosyl-L-methionine + caffeoyl alcohol
-
 Arabidopsis thaliana ?
-
 ?
S-adenosyl-L-methionine + caffeoyl aldehyde
-
 Arabidopsis thaliana ?
-
 ?
S-adenosyl-L-methionine + quercetin
-
 Arabidopsis thaliana ?
-
 ?

Synonyms

Synonyms Comment Organism
AtCOMT1
-
 Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.16
-
 quercetin pH 7.5, temperature not specified in the publication Arabidopsis thaliana
0.62
-
 caffeic acid pH 7.5, temperature not specified in the publication Arabidopsis thaliana
1.3
-
 5-hydroxyferulic acid pH 7.5, temperature not specified in the publication Arabidopsis thaliana
1.52
-
 caffeoyl alcohol pH 7.5, temperature not specified in the publication Arabidopsis thaliana
1.55
-
 caffeoyl aldehyde pH 7.5, temperature not specified in the publication Arabidopsis thaliana
2.23
-
 5-hydroxyconiferyl alcohol pH 7.5, temperature not specified in the publication Arabidopsis thaliana
2.85
-
 5-hydroxyconiferyl aldehyde pH 7.5, temperature not specified in the publication Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Arabidopsis thaliana

General Information

General Information Comment Organism
malfunction Arabidopsis COMT1 knockout mutant line Atomt1 lignin differs from native lignin in wild type plants, in terms of sinapyl (S) alcohol-derived substructures in fiber cell walls being substituted by 5-hydroxyconiferyl alcohol (5OHG)-derived moieties Arabidopsis thaliana