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Literature summary for 2.1.1.68 extracted from
- Purification and properties of S-adenosyl-L-methionine: caffeic acid O-methyltransferase from leaves of spinach beet (Beta vulgaris L) (1975), Biochim. Biophys. Acta, 403, 301-314.
General Stability
| General Stability | Organism |
|---|---|
| 2-mercaptoethanol or dithiothreitol or GSH reduces storage stability | Beta vulgaris |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-Methoxyethanol | - |
Beta vulgaris |  |
| ammonium sulfate | - |
Beta vulgaris | |
| ethanol | - |
Beta vulgaris | |
| Mg2+ | above 4 mM | Beta vulgaris | |
| S-adenosyl-L-homocysteine | strong, product inhibition, competitive | Beta vulgaris | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.013 | - |
S-adenosyl-L-methionine | - |
Beta vulgaris | |
| 0.068 | - |
caffeic acid | - |
Beta vulgaris | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no Mg2+ requirement | Beta vulgaris |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate | Phaseolus vulgaris | i.e. caffeic acid, involved in the biosynthesis of lignin and of some flavonoids in plants | S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate | - |
? | |
| S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate | Beta vulgaris | i.e. caffeic acid, involved in the biosynthesis of lignin and of some flavonoids in plants | S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate | - |
? | |
| S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate | Spinacia oleracea | i.e. caffeic acid, involved in the biosynthesis of lignin and of some flavonoids in plants | S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate | - |
? | |
| S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate | Pisum sativum | i.e. caffeic acid, involved in the biosynthesis of lignin and of some flavonoids in plants | S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Beta vulgaris | - |
ssp. vulgaris, spinach beet | - |
| Phaseolus vulgaris | - |
french bean | - |
| Pisum sativum | - |
pea | - |
| Spinacia oleracea | - |
spinach | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Beta vulgaris |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Beta vulgaris | - |
| leaf | - |
Spinacia oleracea | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0027 | - |
- |
Beta vulgaris |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, at least a month in absence of thiol compounds | Beta vulgaris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate | - |
Phaseolus vulgaris | S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate | - |
? | |
| S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate | - |
Spinacia oleracea | S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate | - |
? | |
| S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate | - |
Pisum sativum | S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate | - |
? | |
| S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate | - |
Beta vulgaris | S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate | i.e. ferulate | ? | |
| S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate | i.e. caffeic acid, involved in the biosynthesis of lignin and of some flavonoids in plants | Phaseolus vulgaris | S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate | - |
? | |
| S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate | i.e. caffeic acid, involved in the biosynthesis of lignin and of some flavonoids in plants | Beta vulgaris | S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate | - |
? | |
| S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate | i.e. caffeic acid, involved in the biosynthesis of lignin and of some flavonoids in plants | Spinacia oleracea | S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate | - |
? | |
| S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate | i.e. caffeic acid, involved in the biosynthesis of lignin and of some flavonoids in plants | Pisum sativum | S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate | - |
? | |
| S-adenosyl-L-methionine + catechol | - |
Beta vulgaris | S-adenosyl-L-homocysteine + 2-methoxyphenol | - |
? | |
| S-adenosyl-L-methionine + protocatechuic acid | - |
Beta vulgaris | S-adenosyl-L-homocysteine + 4-hydroxy-3-methoxybenzoate | - |
? | |
| S-adenosyl-L-methionine + protocatechuic aldehyde | - |
Beta vulgaris | S-adenosyl-L-homocysteine + 4-hydroxy-3-methoxybenzaldehyde | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Beta vulgaris |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
around | Beta vulgaris |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.4 | 7.6 | about half-maximal activity at pH 5.4 and 7.6 | Beta vulgaris |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.004 | - |
S-adenosyl-L-homocysteine | - |
Beta vulgaris | |
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