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Literature summary for 2.1.1.68 extracted from

  • Edwards, R.; Dixon, R.A.
    Purification and characterization of S-adenosyl-L-methionine: caffeic acid 3-O-methyltransferase from suspension cultures of alfalfa (Medicago sativa L.) (1991), Arch. Biochem. Biophys., 287, 372-379.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 7% activity increase at 20 mM Medicago sativa
dithiothreitol 7% activity increase at 20 mM Medicago sativa

Inhibitors

Inhibitors Comment Organism Structure
S-adenosyl-L-homocysteine strong, product inhibition, competitive Medicago sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.007
-
S-adenosyl-L-methionine isozyme II Medicago sativa
0.012
-
S-adenosyl-L-methionine isozyme I Medicago sativa
0.053
-
caffeic acid isozyme I Medicago sativa
0.059
-
caffeic acid isozyme II Medicago sativa

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Medicago sativa
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
SDS-PAGE Medicago sativa

Organism

Organism UniProt Comment Textmining
Medicago sativa
-
elicitor-treated, elicitors are fungal macromolecules from the cell walls of Colletotrichum lindemuthianum, two isoforms
-

Storage Stability

Storage Stability Organism
-70°C, 2 months, stable Medicago sativa
4°C, 4-5 d, assay buffer, 50% activity Medicago sativa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + 3,4-dihydroxy-hydrocinnamic acid
-
Medicago sativa S-adenosyl-L-homocysteine + 4-hydroxy-3-methoxyhydrocinnamic acid
-
?
S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate
-
Medicago sativa S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate i.e. ferulate ?

Subunits

Subunits Comment Organism
monomer 1 * 41000, SDS-PAGE Medicago sativa

Synonyms

Synonyms Comment Organism
COMT
-
Medicago sativa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
-
Medicago sativa

pH Range

pH Minimum pH Maximum Comment Organism
7 7.4 37% of maximal activity at pH 7.0, 75% of maximal activity at pH 7.4 Medicago sativa

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.002
-
S-adenosyl-L-homocysteine isozyme II Medicago sativa
0.004
-
S-adenosyl-L-homocysteine isozyme I Medicago sativa