Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Mycobacterium tuberculosis |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 0.1 M HEPES (pH 7.5), and 6% (w/v) polyethylene glycol 8000 | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
R37L | the mutant exhibits a 10fold-lower affinity for double stranded methylated DNA compared to the wild type enzyme | Mycobacterium tuberculosis |
T15S | the mutant exhibits a 2fold-lower affinity for double stranded methylated DNA compared to the wild type enzyme | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
17844 | - |
x * 17844, calculated from amino acid sequence | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA (containing 6-O-methylguanine) + protein L-cysteine | Mycobacterium tuberculosis | - |
DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WJW5 | - |
- |
Purification (Comment) | Organism |
---|---|
HiTrapQ column chromatography, MonoQ column chromatography, HiTrap heparin column chromatography, and Superdex 200 gel filtration | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA (containing 6-O-methylguanine) + protein L-cysteine | - |
Mycobacterium tuberculosis | DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 17844, calculated from amino acid sequence | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
O6-methylguanine methyltransferase | - |
Mycobacterium tuberculosis |
OGT | - |
Mycobacterium tuberculosis |