Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21-Codon Plus (DE3)-RIL cells | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
21000 | - |
SDS-PAGE | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA containing 6-O-methylguanine + [protein]-L-cysteine | Saccharomyces cerevisiae | - |
DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine | - |
? | |
DNA containing 6-O-methylguanine + [protein]-L-cysteine | Saccharomyces cerevisiae JD52 | - |
DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Saccharomyces cerevisiae JD52 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA resin column chromatography, gel filtration | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA containing 6-O-methylguanine + [protein]-L-cysteine | - |
Saccharomyces cerevisiae | DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine | - |
? | |
DNA containing 6-O-methylguanine + [protein]-L-cysteine | - |
Saccharomyces cerevisiae JD52 | DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alkylguanine transferase | - |
Saccharomyces cerevisiae |
Mgt1 | - |
Saccharomyces cerevisiae |
O6-alkylguanine-DNA alkyltransferase | - |
Saccharomyces cerevisiae |
O6meG-DNA alkyltransferase | - |
Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
metabolism | Mgt1 is a physiological substrate of both the Ubr1-dependent N-end rule pathway and the Ufd4-dependent ubiquitin fusion degradation pathway | Saccharomyces cerevisiae |