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Literature summary for 2.1.1.63 extracted from

  • Hwang, C.S.; Shemorry, A.; Varshavsky, A.
    Two proteolytic pathways regulate DNA repair by cotargeting the Mgt1 alkylguanine transferase (2009), Proc. Natl. Acad. Sci. USA, 106, 2142-2147.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21-Codon Plus (DE3)-RIL cells Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21000
-
SDS-PAGE Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA containing 6-O-methylguanine + [protein]-L-cysteine Saccharomyces cerevisiae
-
DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
-
?
DNA containing 6-O-methylguanine + [protein]-L-cysteine Saccharomyces cerevisiae JD52
-
DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-
Saccharomyces cerevisiae JD52
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA resin column chromatography, gel filtration Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA containing 6-O-methylguanine + [protein]-L-cysteine
-
Saccharomyces cerevisiae DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
-
?
DNA containing 6-O-methylguanine + [protein]-L-cysteine
-
Saccharomyces cerevisiae JD52 DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
-
?

Synonyms

Synonyms Comment Organism
alkylguanine transferase
-
Saccharomyces cerevisiae
Mgt1
-
Saccharomyces cerevisiae
O6-alkylguanine-DNA alkyltransferase
-
Saccharomyces cerevisiae
O6meG-DNA alkyltransferase
-
Saccharomyces cerevisiae

General Information

General Information Comment Organism
metabolism Mgt1 is a physiological substrate of both the Ubr1-dependent N-end rule pathway and the Ufd4-dependent ubiquitin fusion degradation pathway Saccharomyces cerevisiae