Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | no activation by putrescine or spermidine | Escherichia coli | |
NH4+ | - |
Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | strong, 5 mM | Escherichia coli | |
additional information | no inhibition by putrescine or spermidine | Escherichia coli | |
NaCl | mutant trmC1: 100 mM, mutant trmC2: 200 mM | Escherichia coli | |
p-chloromercuribenzoate | - |
Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
NH4+ | activation | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
79000 | - |
1 * 79000, SDS-PAGE | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 5-aminomethyl-2-thiouridine in tRNA | Escherichia coli | - |
S-adenosyl-L-homocysteine + 5-methylaminomethyl-2-thiouridylate in tRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
K12 strain pTH32/1100, harbouring multi-copy plasmid | - |
Purification (Comment) | Organism |
---|---|
affinity chromatography | Escherichia coli |
on Heparin-Sepharose | Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
S-adenosyl-L-methionine + 5-aminomehtyl-2-thiouridine34 in tRNALys = S-adenosyl-L-homocysteine + 5-methylaminomehtyl-2-thiouridine34 in tRNALys | mechanismn | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.1686 | - |
mutant trmC1 | Escherichia coli |
0.9252 | - |
mutant trmC2 | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme exhibits two activities: enzyme from trmC1 mutant reacts with 5-carboxymethylaminomethyl-2-thiouridine to form 5-aminomethyl-2-thiouridine without S-adenosyl-L-methionine, enzyme from trmC2 mutant methylates 5-aminomethyl-2-thiouridine in the presence of S-adenosyl-L-methionine | Escherichia coli | ? | - |
? | |
S-adenosyl-L-methionine + 5-aminomethyl-2-thiouridine in tRNA | - |
Escherichia coli | S-adenosyl-L-homocysteine + 5-methylaminomethyl-2-thiouridylate in tRNA | - |
? | |
S-adenosyl-L-methionine + 5-aminomethyl-2-thiouridine in tRNA | tRNA from E. coli mutants | Escherichia coli | S-adenosyl-L-homocysteine + 5-methylaminomethyl-2-thiouridylate in tRNA | - |
? | |
S-adenosyl-L-methionine + tRNA containing 5-aminomethyl-2-thiouridine | - |
Escherichia coli | S-adenosyl-L-homocysteine + tRNA containing 5-methylaminomethyl-2-thiouridylate | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 79000, SDS-PAGE | Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.23 | - |
tRNA containing 5-aminomethyl-2-thiouridine | - |
Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.5 | - |
Escherichia coli |