Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 Codon Plus (DE3) cells | Rattus norvegicus |
Crystallization (Comment) | Organism |
---|---|
apo and holo forms of rat catechol-O-methyltransferase, hanging drop vapor diffusion method, the apo crystals are grown from 0.2 M (NH4)2SO4, 30% (w/v) PEG8000, the holo form of crystals are grown from 0.2 M (NH4)2SO4, 26% (w/v) PEG8000, 0.2% (v/v) sucrose | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Rattus norvegicus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24889 | - |
1 * 24889, ESI Q-pole mass spectrometry | Rattus norvegicus |
24890 | - |
calculated from amino acid sequence | Rattus norvegicus |
24890 | - |
ESI Q-pole mass spectrometry | Rattus norvegicus |
24891 | - |
1 * 24891, calculated from amino acid sequence | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | P22734 | - |
- |
Purification (Comment) | Organism |
---|---|
GST Sepharose resin column chromatography, GSTrap column chromatography, Super Q-5PW column chromatography, and Superdex 75 gel filtration | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxyestradiol + S-adenosyl-L-methionine | - |
Rattus norvegicus | S-adenosyl-L-homocysteine + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 24889, ESI Q-pole mass spectrometry | Rattus norvegicus |
monomer | 1 * 24891, calculated from amino acid sequence | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
COMT | - |
Rattus norvegicus |
S-COMT | soluble COMT | Rattus norvegicus |