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Literature summary for 2.1.1.6 extracted from
- Solubilization and partial purification of particulate catechol-O-methyltransferase from rat liver (1977), Can. J. Biochem., 55, 1108-1113.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| beta-thujaplicin | 70-100% inhibition at 0.2 mM | Rattus norvegicus |  |
| Mg2+ | required for activity | Rattus norvegicus | |
| N-ethylmaleimide | 70-100% inhibition at 0.2 mM | Rattus norvegicus | |
| p-hydroxymercuribenzoate | 70-100% inhibition at 0.2 mM | Rattus norvegicus | |
| pyrogallol | 70-100% inhibition at 0.2 mM | Rattus norvegicus | |
| tropolone | 70-100% inhibition at 0.2 mM | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Rattus norvegicus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Rattus norvegicus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Rattus norvegicus | |
| Mg2+ | optimum range: 2-5 mM | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 21000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Rattus norvegicus | membrane protein and (or) lipid components may play an important role in catecholamine metabolism | ? | - |
? | |
| S-adenosyl-L-methionine + a catechol | Rattus norvegicus | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
particulate enzyme | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| microsomal | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | membrane protein and (or) lipid components may play an important role in catecholamine metabolism | Rattus norvegicus | ? | - |
? | |
| S-adenosyl-L-methionine + a catechol | - |
Rattus norvegicus | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 8.5 | - |
Rattus norvegicus |
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Release 2023.1 (January 2023)
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