BRENDA - Enzyme Database
show all sequences of 2.1.1.59

Studies on cytochrome c methylation in yeast

DiMaria, P.; Polastro, E.; DeLange, R.J.; Kim, S.; Paik, W.K.; J. Biol. Chem. 254, 4645-4652 (1979)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
S-adenosyl-L-homocysteine
-
Saccharomyces cerevisiae
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.04
-
S-adenosyl-L-methionine
-
Saccharomyces cerevisiae
1.33
-
cytochrome c
of horse heart
Saccharomyces cerevisiae
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
97000
-
gel filtration
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + cytochrome c L-lysine
Saccharomyces cerevisiae
in vivo methylation of cytochrome c in yeast occurs probably before cytochrome c is attached to the mitochondria
S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine
-
Saccharomyces cerevisiae
?
S-adenosyl-L-methionine + cytochrome c L-lysine
Saccharomyces cerevisiae
regulation of enzyme and cytochrome c substrate is not in parallel manner
S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine
-
Saccharomyces cerevisiae
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Saccharomyces cerevisiae
-
-
-
Purification (Commentary)
Commentary
Organism
partially
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.000117
-
partially purified enzyme
Saccharomyces cerevisiae
Storage Stability
Storage Stability
Organism
-20°C, pH 7.0, crude extract supernatant, stable
Saccharomyces cerevisiae
purified enzyme is stabilized by addition of 66% ammonium sulfate during storage
Saccharomyces cerevisiae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + cytochrome c L-lysine
specific for unmethylated cytochrome c
485482
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine
a single lysine residue at position 72 of horse heart cytochrome c is methylated
485482
Saccharomyces cerevisiae
?
S-adenosyl-L-methionine + cytochrome c L-lysine
specific for unmethylated cytochrome c
485482
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine
9:17:74
485482
Saccharomyces cerevisiae
?
S-adenosyl-L-methionine + cytochrome c L-lysine
specific for unmethylated cytochrome c
485482
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine
mixture of epsilon-N-monomethyllysine, epsilon-N-dimethyllysine and epsilon-N-trimethyllysine in the ratio 1:3:4
485482
Saccharomyces cerevisiae
?
S-adenosyl-L-methionine + cytochrome c L-lysine
horse heart cytochrome c-72
485482
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine
a single lysine residue at position 72 of horse heart cytochrome c is methylated
485482
Saccharomyces cerevisiae
?
S-adenosyl-L-methionine + cytochrome c L-lysine
horse heart cytochrome c-72
485482
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine
9:17:74
485482
Saccharomyces cerevisiae
?
S-adenosyl-L-methionine + cytochrome c L-lysine
horse heart cytochrome c-72
485482
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine
mixture of epsilon-N-monomethyllysine, epsilon-N-dimethyllysine and epsilon-N-trimethyllysine in the ratio 1:3:4
485482
Saccharomyces cerevisiae
?
S-adenosyl-L-methionine + cytochrome c L-lysine
overview: substrate activity of various cytochromes c
485482
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine
a single lysine residue at position 72 of horse heart cytochrome c is methylated
485482
Saccharomyces cerevisiae
?
S-adenosyl-L-methionine + cytochrome c L-lysine
overview: substrate activity of various cytochromes c
485482
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine
9:17:74
485482
Saccharomyces cerevisiae
?
S-adenosyl-L-methionine + cytochrome c L-lysine
overview: substrate activity of various cytochromes c
485482
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine
mixture of epsilon-N-monomethyllysine, epsilon-N-dimethyllysine and epsilon-N-trimethyllysine in the ratio 1:3:4
485482
Saccharomyces cerevisiae
?
S-adenosyl-L-methionine + cytochrome c L-lysine
in vivo methylation of cytochrome c in yeast occurs probably before cytochrome c is attached to the mitochondria
485482
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine
-
485482
Saccharomyces cerevisiae
?
S-adenosyl-L-methionine + cytochrome c L-lysine
regulation of enzyme and cytochrome c substrate is not in parallel manner
485482
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine
-
485482
Saccharomyces cerevisiae
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
-
Saccharomyces cerevisiae
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
S-adenosyl-L-homocysteine
-
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.04
-
S-adenosyl-L-methionine
-
Saccharomyces cerevisiae
1.33
-
cytochrome c
of horse heart
Saccharomyces cerevisiae
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
97000
-
gel filtration
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + cytochrome c L-lysine
Saccharomyces cerevisiae
in vivo methylation of cytochrome c in yeast occurs probably before cytochrome c is attached to the mitochondria
S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine
-
Saccharomyces cerevisiae
?
S-adenosyl-L-methionine + cytochrome c L-lysine
Saccharomyces cerevisiae
regulation of enzyme and cytochrome c substrate is not in parallel manner
S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine
-
Saccharomyces cerevisiae
?
Purification (Commentary) (protein specific)
Commentary
Organism
partially
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.000117
-
partially purified enzyme
Saccharomyces cerevisiae
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, pH 7.0, crude extract supernatant, stable
Saccharomyces cerevisiae
purified enzyme is stabilized by addition of 66% ammonium sulfate during storage
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + cytochrome c L-lysine
specific for unmethylated cytochrome c
485482
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine
a single lysine residue at position 72 of horse heart cytochrome c is methylated
485482
Saccharomyces cerevisiae
?
S-adenosyl-L-methionine + cytochrome c L-lysine
specific for unmethylated cytochrome c
485482
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine
9:17:74
485482
Saccharomyces cerevisiae
?
S-adenosyl-L-methionine + cytochrome c L-lysine
specific for unmethylated cytochrome c
485482
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine
mixture of epsilon-N-monomethyllysine, epsilon-N-dimethyllysine and epsilon-N-trimethyllysine in the ratio 1:3:4
485482
Saccharomyces cerevisiae
?
S-adenosyl-L-methionine + cytochrome c L-lysine
horse heart cytochrome c-72
485482
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine
a single lysine residue at position 72 of horse heart cytochrome c is methylated
485482
Saccharomyces cerevisiae
?
S-adenosyl-L-methionine + cytochrome c L-lysine
horse heart cytochrome c-72
485482
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine
9:17:74
485482
Saccharomyces cerevisiae
?
S-adenosyl-L-methionine + cytochrome c L-lysine
horse heart cytochrome c-72
485482
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine
mixture of epsilon-N-monomethyllysine, epsilon-N-dimethyllysine and epsilon-N-trimethyllysine in the ratio 1:3:4
485482
Saccharomyces cerevisiae
?
S-adenosyl-L-methionine + cytochrome c L-lysine
overview: substrate activity of various cytochromes c
485482
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine
a single lysine residue at position 72 of horse heart cytochrome c is methylated
485482
Saccharomyces cerevisiae
?
S-adenosyl-L-methionine + cytochrome c L-lysine
overview: substrate activity of various cytochromes c
485482
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine
9:17:74
485482
Saccharomyces cerevisiae
?
S-adenosyl-L-methionine + cytochrome c L-lysine
overview: substrate activity of various cytochromes c
485482
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine
mixture of epsilon-N-monomethyllysine, epsilon-N-dimethyllysine and epsilon-N-trimethyllysine in the ratio 1:3:4
485482
Saccharomyces cerevisiae
?
S-adenosyl-L-methionine + cytochrome c L-lysine
in vivo methylation of cytochrome c in yeast occurs probably before cytochrome c is attached to the mitochondria
485482
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine
-
485482
Saccharomyces cerevisiae
?
S-adenosyl-L-methionine + cytochrome c L-lysine
regulation of enzyme and cytochrome c substrate is not in parallel manner
485482
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine
-
485482
Saccharomyces cerevisiae
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
-
Saccharomyces cerevisiae
Other publictions for EC 2.1.1.59
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
485478
Polevoda
Cytochrome c methyltransferase ...
Saccharomyces cerevisiae
J. Biol. Chem.
275
20508-20513
2000
-
-
1
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
485477
Park
Enzymatic methylation of in vi ...
Saccharomyces cerevisiae
J. Biol. Chem.
262
14702-14708
1987
-
-
1
-
-
-
2
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
485480
Paik
Enzymatic methylation and deme ...
Neurospora crassa
Methods Enzymol.
106
274-287
1984
-
-
-
-
-
-
2
7
2
-
1
1
-
1
-
-
1
-
-
1
1
1
5
-
1
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
1
7
2
-
1
1
-
-
-
1
-
1
1
1
5
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
485479
Durban
-
Cytochrome c-specific protein- ...
Neurospora crassa
Korean J. Biochem.
15
19-24
1983
-
-
-
-
-
-
2
2
-
-
-
1
-
1
-
-
1
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
1
2
-
-
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
485474
Valentine
A cytochrome c methyltransfera ...
Strigomonas oncopelti
Biochem. J.
201
329-338
1982
-
-
-
-
-
-
-
-
-
-
-
1
-
6
-
-
-
1
-
-
1
-
3
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
3
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
485481
DiMaria
Cytochrome c specific methylas ...
Triticum aestivum
Biochemistry
21
1036-1044
1982
-
-
-
-
-
-
2
2
-
-
-
1
-
1
-
-
1
-
-
1
2
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
1
-
-
-
1
-
1
2
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
485482
DiMaria
Studies on cytochrome c methyl ...
Saccharomyces cerevisiae
J. Biol. Chem.
254
4645-4652
1979
-
-
-
-
-
-
1
2
-
-
1
2
-
1
-
-
1
-
-
-
1
2
11
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
1
2
-
-
-
1
-
-
1
2
11
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
485476
Durban
Cytochrome c-specific protein- ...
Neurospora crassa
J. Biol. Chem.
253
1427-1435
1978
-
-
-
-
-
-
2
7
1
-
1
1
-
2
-
-
1
1
-
-
1
-
11
-
1
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
1
7
1
-
1
1
-
-
-
1
-
-
1
-
11
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
485475
Nochumson
Cytochrome c-specific protein ...
Neurospora crassa
Biochem. J.
165
11-18
1977
-
-
-
-
-
-
-
2
3
-
-
1
-
3
-
-
1
1
-
1
1
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
3
-
-
1
-
-
-
1
-
1
1
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-