BRENDA - Enzyme Database
show all sequences of 2.1.1.59

Cytochrome c-specific protein-lysine methyltransferase from Neurospora crassa. Purification, characterization, and substrate requirements

Durban, E.; Nochumson, S.; Kim, S.; Ki Paik, W.; J. Biol. Chem. 253, 1427-1435 (1978)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
S-adenosyl-L-homocysteine
competitive versus S-adenosyl-L-methionine
Neurospora crassa
S-adenosyl-L-methionine
-
Neurospora crassa
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.007
-
CNBr peptide
residue 1-80 of horse heart cytochrome c
Neurospora crassa
0.03
-
cytochrome c
of horse heart, heme-free
Neurospora crassa
0.04
-
CNBr peptide
residue 1-65 or residue 66-104 of horse heart cytochrome c
Neurospora crassa
0.2
-
cytochrome c
of horse heart, ethanol-denatured
Neurospora crassa
0.32
-
cytochrome c
of horse heart, native
Neurospora crassa
1.7
-
cytochrome c
-
Neurospora crassa
19
-
S-adenosyl-L-methionine
-
Neurospora crassa
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Neurospora crassa
5829
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
120000
-
gel filtration
Neurospora crassa
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + cytochrome c L-lysine
Neurospora crassa
-
S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine
-
Neurospora crassa
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Neurospora crassa
-
-
-
Purification (Commentary)
Commentary
Organism
-
Neurospora crassa
Reaction
Reaction
Commentary
Organism
S-adenosyl-L-methionine + [cytochrome c]-L-lysine = S-adenosyl-L-homocysteine + [cytochrome c]-N6-methyl-L-lysine
specific methylation site, 1 lysine in cytochrome c, comparison
Neurospora crassa
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.0295
-
purified enzyme
Neurospora crassa
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + cytochrome c L-lysine
-
485476
Neurospora crassa
S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine
-
485476
Neurospora crassa
?
S-adenosyl-L-methionine + cytochrome c L-lysine
horse heart cytochrome c-72
485476
Neurospora crassa
S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine
a single lysine residue at position 72 of horse heart cytochrome c is methylated
485476
Neurospora crassa
?
S-adenosyl-L-methionine + cytochrome c L-lysine
horse heart cytochrome c-72
485476
Neurospora crassa
S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine
mixture of epsilon-N-monomethyllysine, epsilon-N-dimethyllysine and epsilon-N-trimethyllysine in the ratio 1:3:4
485476
Neurospora crassa
?
S-adenosyl-L-methionine + cytochrome c L-lysine
important features in determining methyl-accepting activity: 1. reactive site such as-X-Lys-Lys-Y-, 2. length of polypeptide chain, 3. tertiary structure
485476
Neurospora crassa
S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine
a single lysine residue at position 72 of horse heart cytochrome c is methylated
485476
Neurospora crassa
?
S-adenosyl-L-methionine + cytochrome c L-lysine
important features in determining methyl-accepting activity: 1. reactive site such as-X-Lys-Lys-Y-, 2. length of polypeptide chain, 3. tertiary structure
485476
Neurospora crassa
S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine
mixture of epsilon-N-monomethyllysine, epsilon-N-dimethyllysine and epsilon-N-trimethyllysine in the ratio 1:3:4
485476
Neurospora crassa
?
S-adenosyl-L-methionine + cytochrome c L-lysine
does not randomly methylate cytochrome c, but shows absolute specificity for some amino acid sequences
485476
Neurospora crassa
S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine
a single lysine residue at position 72 of horse heart cytochrome c is methylated
485476
Neurospora crassa
?
S-adenosyl-L-methionine + cytochrome c L-lysine
does not randomly methylate cytochrome c, but shows absolute specificity for some amino acid sequences
485476
Neurospora crassa
S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine
mixture of epsilon-N-monomethyllysine, epsilon-N-dimethyllysine and epsilon-N-trimethyllysine in the ratio 1:3:4
485476
Neurospora crassa
?
S-adenosyl-L-methionine + cytochrome c L-lysine
also CNBr peptides of horse heart cytochrome c can serve as substrates, overview
485476
Neurospora crassa
S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine
a single lysine residue at position 72 of horse heart cytochrome c is methylated
485476
Neurospora crassa
?
S-adenosyl-L-methionine + cytochrome c L-lysine
also CNBr peptides of horse heart cytochrome c can serve as substrates, overview
485476
Neurospora crassa
S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine
mixture of epsilon-N-monomethyllysine, epsilon-N-dimethyllysine and epsilon-N-trimethyllysine in the ratio 1:3:4
485476
Neurospora crassa
?
S-adenosyl-L-methionine + cytochrome c L-lysine
overview: substrate activity of various cytochromes c
485476
Neurospora crassa
S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine
a single lysine residue at position 72 of horse heart cytochrome c is methylated
485476
Neurospora crassa
?
S-adenosyl-L-methionine + cytochrome c L-lysine
overview: substrate activity of various cytochromes c
485476
Neurospora crassa
S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine
mixture of epsilon-N-monomethyllysine, epsilon-N-dimethyllysine and epsilon-N-trimethyllysine in the ratio 1:3:4
485476
Neurospora crassa
?
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Neurospora crassa
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
-
Neurospora crassa
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.002
-
S-adenosyl-L-methionine
-
Neurospora crassa
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
S-adenosyl-L-homocysteine
competitive versus S-adenosyl-L-methionine
Neurospora crassa
S-adenosyl-L-methionine
-
Neurospora crassa
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.002
-
S-adenosyl-L-methionine
-
Neurospora crassa
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.007
-
CNBr peptide
residue 1-80 of horse heart cytochrome c
Neurospora crassa
0.03
-
cytochrome c
of horse heart, heme-free
Neurospora crassa
0.04
-
CNBr peptide
residue 1-65 or residue 66-104 of horse heart cytochrome c
Neurospora crassa
0.2
-
cytochrome c
of horse heart, ethanol-denatured
Neurospora crassa
0.32
-
cytochrome c
of horse heart, native
Neurospora crassa
1.7
-
cytochrome c
-
Neurospora crassa
19
-
S-adenosyl-L-methionine
-
Neurospora crassa
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Neurospora crassa
5829
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
120000
-
gel filtration
Neurospora crassa
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + cytochrome c L-lysine
Neurospora crassa
-
S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine
-
Neurospora crassa
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Neurospora crassa
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.0295
-
purified enzyme
Neurospora crassa
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + cytochrome c L-lysine
-
485476
Neurospora crassa
S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine
-
485476
Neurospora crassa
?
S-adenosyl-L-methionine + cytochrome c L-lysine
horse heart cytochrome c-72
485476
Neurospora crassa
S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine
a single lysine residue at position 72 of horse heart cytochrome c is methylated
485476
Neurospora crassa
?
S-adenosyl-L-methionine + cytochrome c L-lysine
horse heart cytochrome c-72
485476
Neurospora crassa
S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine
mixture of epsilon-N-monomethyllysine, epsilon-N-dimethyllysine and epsilon-N-trimethyllysine in the ratio 1:3:4
485476
Neurospora crassa
?
S-adenosyl-L-methionine + cytochrome c L-lysine
important features in determining methyl-accepting activity: 1. reactive site such as-X-Lys-Lys-Y-, 2. length of polypeptide chain, 3. tertiary structure
485476
Neurospora crassa
S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine
a single lysine residue at position 72 of horse heart cytochrome c is methylated
485476
Neurospora crassa
?
S-adenosyl-L-methionine + cytochrome c L-lysine
important features in determining methyl-accepting activity: 1. reactive site such as-X-Lys-Lys-Y-, 2. length of polypeptide chain, 3. tertiary structure
485476
Neurospora crassa
S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine
mixture of epsilon-N-monomethyllysine, epsilon-N-dimethyllysine and epsilon-N-trimethyllysine in the ratio 1:3:4
485476
Neurospora crassa
?
S-adenosyl-L-methionine + cytochrome c L-lysine
does not randomly methylate cytochrome c, but shows absolute specificity for some amino acid sequences
485476
Neurospora crassa
S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine
a single lysine residue at position 72 of horse heart cytochrome c is methylated
485476
Neurospora crassa
?
S-adenosyl-L-methionine + cytochrome c L-lysine
does not randomly methylate cytochrome c, but shows absolute specificity for some amino acid sequences
485476
Neurospora crassa
S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine
mixture of epsilon-N-monomethyllysine, epsilon-N-dimethyllysine and epsilon-N-trimethyllysine in the ratio 1:3:4
485476
Neurospora crassa
?
S-adenosyl-L-methionine + cytochrome c L-lysine
also CNBr peptides of horse heart cytochrome c can serve as substrates, overview
485476
Neurospora crassa
S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine
a single lysine residue at position 72 of horse heart cytochrome c is methylated
485476
Neurospora crassa
?
S-adenosyl-L-methionine + cytochrome c L-lysine
also CNBr peptides of horse heart cytochrome c can serve as substrates, overview
485476
Neurospora crassa
S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine
mixture of epsilon-N-monomethyllysine, epsilon-N-dimethyllysine and epsilon-N-trimethyllysine in the ratio 1:3:4
485476
Neurospora crassa
?
S-adenosyl-L-methionine + cytochrome c L-lysine
overview: substrate activity of various cytochromes c
485476
Neurospora crassa
S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine
a single lysine residue at position 72 of horse heart cytochrome c is methylated
485476
Neurospora crassa
?
S-adenosyl-L-methionine + cytochrome c L-lysine
overview: substrate activity of various cytochromes c
485476
Neurospora crassa
S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine
mixture of epsilon-N-monomethyllysine, epsilon-N-dimethyllysine and epsilon-N-trimethyllysine in the ratio 1:3:4
485476
Neurospora crassa
?
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Neurospora crassa
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
-
Neurospora crassa
Other publictions for EC 2.1.1.59
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
485478
Polevoda
Cytochrome c methyltransferase ...
Saccharomyces cerevisiae
J. Biol. Chem.
275
20508-20513
2000
-
-
1
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
485477
Park
Enzymatic methylation of in vi ...
Saccharomyces cerevisiae
J. Biol. Chem.
262
14702-14708
1987
-
-
1
-
-
-
2
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
485480
Paik
Enzymatic methylation and deme ...
Neurospora crassa
Methods Enzymol.
106
274-287
1984
-
-
-
-
-
-
2
7
2
-
1
1
-
1
-
-
1
-
-
1
1
1
5
-
1
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
1
7
2
-
1
1
-
-
-
1
-
1
1
1
5
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
485479
Durban
-
Cytochrome c-specific protein- ...
Neurospora crassa
Korean J. Biochem.
15
19-24
1983
-
-
-
-
-
-
2
2
-
-
-
1
-
1
-
-
1
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
1
2
-
-
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
485474
Valentine
A cytochrome c methyltransfera ...
Strigomonas oncopelti
Biochem. J.
201
329-338
1982
-
-
-
-
-
-
-
-
-
-
-
1
-
6
-
-
-
1
-
-
1
-
3
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
3
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
485481
DiMaria
Cytochrome c specific methylas ...
Triticum aestivum
Biochemistry
21
1036-1044
1982
-
-
-
-
-
-
2
2
-
-
-
1
-
1
-
-
1
-
-
1
2
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
1
-
-
-
1
-
1
2
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
485482
DiMaria
Studies on cytochrome c methyl ...
Saccharomyces cerevisiae
J. Biol. Chem.
254
4645-4652
1979
-
-
-
-
-
-
1
2
-
-
1
2
-
1
-
-
1
-
-
-
1
2
11
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
1
2
-
-
-
1
-
-
1
2
11
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
485476
Durban
Cytochrome c-specific protein- ...
Neurospora crassa
J. Biol. Chem.
253
1427-1435
1978
-
-
-
-
-
-
2
7
1
-
1
1
-
2
-
-
1
1
-
-
1
-
11
-
1
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
1
7
1
-
1
1
-
-
-
1
-
-
1
-
11
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
485475
Nochumson
Cytochrome c-specific protein ...
Neurospora crassa
Biochem. J.
165
11-18
1977
-
-
-
-
-
-
-
2
3
-
-
1
-
3
-
-
1
1
-
1
1
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
3
-
-
1
-
-
-
1
-
1
1
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-