BRENDA - Enzyme Database
show all sequences of 2.1.1.59

Cytochrome c-specific protein methylase III from Neurospora crassa

Nochumson, S.; Durban, E.; Kim, S.; Paik, W.K.; Biochem. J. 165, 11-18 (1977)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0095
-
S-adenosyl-L-methionine
-
Neurospora crassa
0.12
-
cytochrome c
-
Neurospora crassa
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
74.3% of the enzyme activity
Neurospora crassa
5829
-
lipid bilayer
13.6% of the enzyme activity
Neurospora crassa
-
-
membrane
-
Neurospora crassa
16020
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + cytochrome c L-lysine
Neurospora crassa
-
S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine
-
Neurospora crassa
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Neurospora crassa
-
-
-
Purification (Commentary)
Commentary
Organism
partially
Neurospora crassa
Reaction
Reaction
Commentary
Organism
S-adenosyl-L-methionine + [cytochrome c]-L-lysine = S-adenosyl-L-homocysteine + [cytochrome c]-N6-methyl-L-lysine
specific methylation site, 1 lysine in cytochrome c, comparison
Neurospora crassa
Source Tissue
Source Tissue
Commentary
Organism
Textmining
mycelium
-
Neurospora crassa
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.000289
-
partially purified enzyme
Neurospora crassa
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + cytochrome c L-lysine
-
485475
Neurospora crassa
S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine
-
485475
Neurospora crassa
?
S-adenosyl-L-methionine + cytochrome c L-lysine
horse heart cytochrome c-72
485475
Neurospora crassa
S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine
mixture of epsilon-N-monomethyllysine, epsilon-N-dimethyllysine and epsilon-N-trimethyllysine in the ratio 1:3:4
485475
Neurospora crassa
?
S-adenosyl-L-methionine + cytochrome c L-lysine
does not randomly methylate cytochrome c, but shows absolute specificity for some amino acid sequences
485475
Neurospora crassa
S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine
mixture of epsilon-N-monomethyllysine, epsilon-N-dimethyllysine and epsilon-N-trimethyllysine in the ratio 1:3:4
485475
Neurospora crassa
?
S-adenosyl-L-methionine + myoglobin L-lysine
10% activity compared to cytochrome c
485475
Neurospora crassa
S-adenosyl-L-homocysteine + myoglobin N6-methyl-L-lysine
-
485475
Neurospora crassa
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
-
Neurospora crassa
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0095
-
S-adenosyl-L-methionine
-
Neurospora crassa
0.12
-
cytochrome c
-
Neurospora crassa
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
74.3% of the enzyme activity
Neurospora crassa
5829
-
lipid bilayer
13.6% of the enzyme activity
Neurospora crassa
-
-
membrane
-
Neurospora crassa
16020
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + cytochrome c L-lysine
Neurospora crassa
-
S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine
-
Neurospora crassa
?
Purification (Commentary) (protein specific)
Commentary
Organism
partially
Neurospora crassa
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
mycelium
-
Neurospora crassa
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.000289
-
partially purified enzyme
Neurospora crassa
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + cytochrome c L-lysine
-
485475
Neurospora crassa
S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine
-
485475
Neurospora crassa
?
S-adenosyl-L-methionine + cytochrome c L-lysine
horse heart cytochrome c-72
485475
Neurospora crassa
S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine
mixture of epsilon-N-monomethyllysine, epsilon-N-dimethyllysine and epsilon-N-trimethyllysine in the ratio 1:3:4
485475
Neurospora crassa
?
S-adenosyl-L-methionine + cytochrome c L-lysine
does not randomly methylate cytochrome c, but shows absolute specificity for some amino acid sequences
485475
Neurospora crassa
S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine
mixture of epsilon-N-monomethyllysine, epsilon-N-dimethyllysine and epsilon-N-trimethyllysine in the ratio 1:3:4
485475
Neurospora crassa
?
S-adenosyl-L-methionine + myoglobin L-lysine
10% activity compared to cytochrome c
485475
Neurospora crassa
S-adenosyl-L-homocysteine + myoglobin N6-methyl-L-lysine
-
485475
Neurospora crassa
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
-
Neurospora crassa
Other publictions for EC 2.1.1.59
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
485478
Polevoda
Cytochrome c methyltransferase ...
Saccharomyces cerevisiae
J. Biol. Chem.
275
20508-20513
2000
-
-
1
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
485477
Park
Enzymatic methylation of in vi ...
Saccharomyces cerevisiae
J. Biol. Chem.
262
14702-14708
1987
-
-
1
-
-
-
2
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
485480
Paik
Enzymatic methylation and deme ...
Neurospora crassa
Methods Enzymol.
106
274-287
1984
-
-
-
-
-
-
2
7
2
-
1
1
-
1
-
-
1
-
-
1
1
1
5
-
1
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
1
7
2
-
1
1
-
-
-
1
-
1
1
1
5
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
485479
Durban
-
Cytochrome c-specific protein- ...
Neurospora crassa
Korean J. Biochem.
15
19-24
1983
-
-
-
-
-
-
2
2
-
-
-
1
-
1
-
-
1
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
1
2
-
-
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
485474
Valentine
A cytochrome c methyltransfera ...
Strigomonas oncopelti
Biochem. J.
201
329-338
1982
-
-
-
-
-
-
-
-
-
-
-
1
-
6
-
-
-
1
-
-
1
-
3
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
3
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
485481
DiMaria
Cytochrome c specific methylas ...
Triticum aestivum
Biochemistry
21
1036-1044
1982
-
-
-
-
-
-
2
2
-
-
-
1
-
1
-
-
1
-
-
1
2
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
1
-
-
-
1
-
1
2
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
485482
DiMaria
Studies on cytochrome c methyl ...
Saccharomyces cerevisiae
J. Biol. Chem.
254
4645-4652
1979
-
-
-
-
-
-
1
2
-
-
1
2
-
1
-
-
1
-
-
-
1
2
11
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
1
2
-
-
-
1
-
-
1
2
11
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
485476
Durban
Cytochrome c-specific protein- ...
Neurospora crassa
J. Biol. Chem.
253
1427-1435
1978
-
-
-
-
-
-
2
7
1
-
1
1
-
2
-
-
1
1
-
-
1
-
11
-
1
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
1
7
1
-
1
1
-
-
-
1
-
-
1
-
11
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
485475
Nochumson
Cytochrome c-specific protein ...
Neurospora crassa
Biochem. J.
165
11-18
1977
-
-
-
-
-
-
-
2
3
-
-
1
-
3
-
-
1
1
-
1
1
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
3
-
-
1
-
-
-
1
-
1
1
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-