Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli, His-tagged | Xenopus laevis |
expression in Escherichia coli, His-tagged, amino acid sequence analysis | Xenopus laevis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
Western blot | Xenopus laevis |
58660 | 60000 | DNA sequence determination, western blot | Xenopus laevis |
68820 | 70000 | DNA sequence determination, western blot | Xenopus laevis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Xenopus laevis | methylation of the cap structure is also implicated in polyadenylation-mediated translational activation during Xenopus oocyte maturation | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Xenopus laevis | Q9I8S2 | fragment of 402 amino acids | - |
Xenopus laevis | Q9IA92 | mRNA (guanine-7-)methyltransferase component of the capping enzyme | - |
Xenopus laevis | Q9IA93 | mRNAcapping enzyme | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N7-methyl 5'-triphosphoguanosine)-[mRNA] | RNA triphosphatase, RNA guanylyltransferase, and RNA (guanine-N7-)methyltransferase form the mRNA capping enzyme complex consiting of a small and a large subunit | Xenopus laevis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
zygote | - |
Xenopus laevis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | methylation of the cap structure is also implicated in polyadenylation-mediated translational activation during Xenopus oocyte maturation | Xenopus laevis | ? | - |
? | |
S-adenosyl-L-methionine + G(5')pppR-RNA | - |
Xenopus laevis | S-adenosyl-L-homocysteine + m7G(5')pppR-RNA | - |
? |