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Literature summary for 2.1.1.56 extracted from

  • Locht, C.; Beaudart, J.L.; Delcour, J.
    Partial purification and characterization of mRNA (guanine-7-) methyltransferase from the yeast Saccharomyces cerevisiae (1983), Eur. J. Biochem., 134, 117-121.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ strong Saccharomyces cerevisiae
Mg2+ strong Saccharomyces cerevisiae
Mn2+ strong Saccharomyces cerevisiae
additional information inhibition by a natural low-molecular-weight inhibitor in the crude extract, removed during purification Saccharomyces cerevisiae
S-adenosyl-L-homocysteine product inhibition, competitive Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
S-adenosyl-L-methionine
-
Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
KCl no activation Saccharomyces cerevisiae
Mg2+ inactivation Saccharomyces cerevisiae
Mn2+ inhibition Saccharomyces cerevisiae
additional information no activation by NaCl Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49000
-
gel filtration Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
osmotic sensitive
-
Saccharomyces cerevisiae VY1160
-
osmotic sensitive
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharomyces cerevisiae

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture
-
Saccharomyces cerevisiae
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00000235
-
purified enzyme Saccharomyces cerevisiae

Storage Stability

Storage Stability Organism
-80°C, several months Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + G(5')pppR-RNA methylates also undermethylated poly(A)-rich mRNA Saccharomyces cerevisiae S-adenosyl-L-homocysteine + m7G(5')pppR-RNA R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap ?
S-adenosyl-L-methionine + G(5')pppR-RNA methylates also undermethylated poly(A)-rich mRNA Saccharomyces cerevisiae VY1160 S-adenosyl-L-homocysteine + m7G(5')pppR-RNA R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap ?

Subunits

Subunits Comment Organism
monomer 1 * 49000, SDS-PAGE Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
around Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Saccharomyces cerevisiae

pH Range

pH Minimum pH Maximum Comment Organism
5 8 half-maximal activity at pH 5.0, unstable above pH 8.0 Saccharomyces cerevisiae