Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | 70% of total activity, 1/3 of which is losely associated to ribosomes | Homo sapiens | 5737 | - |
nucleus | 30% of total activity | Homo sapiens | 5634 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | - |
Homo sapiens | |
Mg2+ | activation | Homo sapiens | |
additional information | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
gel filtration, sucrose density gradient centrifugation | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
HeLa cells | - |
Purification (Comment) | Organism |
---|---|
DNA-agarose affinity chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell suspension culture | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.000593 | - |
purified enzyme | Homo sapiens |
Storage Stability | Organism |
---|---|
-80°C, several months | Homo sapiens |
4°C, t1/2: less than 36 h | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no substrates are poly-(A) terminating in a single phosphate or a diphosphate | Homo sapiens | ? | - |
? | |
additional information | GDP, GTP, G(5')ppppG | Homo sapiens | ? | - |
? | |
S-adenosyl-L-methionine + G(5')pppN | N: G | Homo sapiens | S-adenosyl-L-homocysteine + m7G(5')pppG | - |
? | |
S-adenosyl-L-methionine + G(5')pppR-RNA | mRNA, synthetic polyribonucleotides terminating in a dinucleoside triphosphate | Homo sapiens | S-adenosyl-L-homocysteine + m7G(5')pppR-RNA | R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap | ? | |
S-adenosyl-L-methionine + G(5')pppR-RNA | specific for mRNAs with 5'-terminal sequence G(5')pppA- and G(5')pppG- | Homo sapiens | S-adenosyl-L-homocysteine + m7G(5')pppR-RNA | R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap | ? | |
S-adenosyl-L-methionine + G(5')pppR-RNA | R may be guanosine or adenosine, methyl acceptors are unmethylated vaccinia virus mRNAs | Homo sapiens | S-adenosyl-L-homocysteine + m7G(5')pppR-RNA | R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap | ? | |
S-adenosyl-L-methionine + G(5')pppR-RNA | active also on E. coli tRNA | Homo sapiens | S-adenosyl-L-homocysteine + m7G(5')pppR-RNA | R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap | ? | |
S-adenosyl-L-methionine + G(5')pppR-RNA | free G(5')pppG is utilized, G(5')ppG is a poor substrate, while GTP, GDP, and G(5')ppppG are no substrates | Homo sapiens | S-adenosyl-L-homocysteine + m7G(5')pppR-RNA | R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap | ? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | 7.8 | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no requirement of GTP | Homo sapiens |