BRENDA - Enzyme Database
show all sequences of 2.1.1.54

Characterization of deoxycytidylate methyltransferase in Xanthomonas oryzae infected with bacteriophage Xp12

Feng, T.Y.; Tu, J.; Kuo, T.T.; Eur. J. Biochem. 87, 29-36 (1978)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Cu2+
above 1 mM
Xanthomonas oryzae
Mg2+
above 50 mM
Xanthomonas oryzae
Zn2+
above 1 mM
Xanthomonas oryzae
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0313
-
dCMP
-
Xanthomonas oryzae
0.0714
-
tetrahydrofolic acid
-
Xanthomonas oryzae
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
25 mM, slight stimulation
Xanthomonas oryzae
Cu2+
below 1 mM, stimulation
Xanthomonas oryzae
K+
150 mM, stimulation
Xanthomonas oryzae
Mg2+
stimulates below 25 mM
Xanthomonas oryzae
Mn2+
has a similar effect in stimulation but not as strong as Mg2+
Xanthomonas oryzae
additional information
among the buffers tested, 6-morpholinoethane sulfonate/Mg2+ is the best
Xanthomonas oryzae
Zn2+
below 1 mM, stimulation
Xanthomonas oryzae
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Xanthomonas oryzae
-
phage Xp12-infected
-
Purification (Commentary)
Commentary
Organism
phage Xp12-infected, partial
Xanthomonas oryzae
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.11
-
-
Xanthomonas oryzae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5,10-methylenetetrahydrofolate + CMP
-
441229
Xanthomonas oryzae
dihydrofolate + 5-methylcytidylate
-
-
-
?
5,10-methylenetetrahydrofolate + CTP
low activity
441229
Xanthomonas oryzae
dihydrofolate + 5-methylcytidine 5'-triphosphate
-
-
-
-
5,10-methylenetetrahydrofolate + dCMP
-
441229
Xanthomonas oryzae
dihydrofolate + deoxy-5-methylcytidylate
-
-
-
-
5,10-methylenetetrahydrofolate + dCTP
low activity
441229
Xanthomonas oryzae
dihydrofolate + deoxy-5-methylcytidine 5'-triphosphate
-
-
-
?
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
assay at
Xanthomonas oryzae
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
50
-
3 min, 90% loss of activity
Xanthomonas oryzae
70
-
3 min, complete loss of activity
Xanthomonas oryzae
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
-
Xanthomonas oryzae
pH Range
pH Minimum
pH Maximum
Commentary
Organism
additional information
-
-
Xanthomonas oryzae
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Cu2+
above 1 mM
Xanthomonas oryzae
Mg2+
above 50 mM
Xanthomonas oryzae
Zn2+
above 1 mM
Xanthomonas oryzae
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0313
-
dCMP
-
Xanthomonas oryzae
0.0714
-
tetrahydrofolic acid
-
Xanthomonas oryzae
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
25 mM, slight stimulation
Xanthomonas oryzae
Cu2+
below 1 mM, stimulation
Xanthomonas oryzae
K+
150 mM, stimulation
Xanthomonas oryzae
Mg2+
stimulates below 25 mM
Xanthomonas oryzae
Mn2+
has a similar effect in stimulation but not as strong as Mg2+
Xanthomonas oryzae
additional information
among the buffers tested, 6-morpholinoethane sulfonate/Mg2+ is the best
Xanthomonas oryzae
Zn2+
below 1 mM, stimulation
Xanthomonas oryzae
Purification (Commentary) (protein specific)
Commentary
Organism
phage Xp12-infected, partial
Xanthomonas oryzae
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.11
-
-
Xanthomonas oryzae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5,10-methylenetetrahydrofolate + CMP
-
441229
Xanthomonas oryzae
dihydrofolate + 5-methylcytidylate
-
-
-
?
5,10-methylenetetrahydrofolate + CTP
low activity
441229
Xanthomonas oryzae
dihydrofolate + 5-methylcytidine 5'-triphosphate
-
-
-
-
5,10-methylenetetrahydrofolate + dCMP
-
441229
Xanthomonas oryzae
dihydrofolate + deoxy-5-methylcytidylate
-
-
-
-
5,10-methylenetetrahydrofolate + dCTP
low activity
441229
Xanthomonas oryzae
dihydrofolate + deoxy-5-methylcytidine 5'-triphosphate
-
-
-
?
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
assay at
Xanthomonas oryzae
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
50
-
3 min, 90% loss of activity
Xanthomonas oryzae
70
-
3 min, complete loss of activity
Xanthomonas oryzae
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
-
Xanthomonas oryzae
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
additional information
-
-
Xanthomonas oryzae
Other publictions for EC 2.1.1.54
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
441228
Wang
Enzymatic conversion of deoxyc ...
Xanthomonas oryzae
Anal. Biochem.
122
89-93
1982
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-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
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-
-
-
-
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2
-
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-
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-
-
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-
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-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
441229
Feng
Characterization of deoxycytid ...
Xanthomonas oryzae
Eur. J. Biochem.
87
29-36
1978
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-
-
-
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3
2
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7
-
-
-
2
-
-
1
-
-
-
1
-
4
-
1
-
2
-
1
1
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-
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-
-
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-
-
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-
-
-
-
3
-
2
-
7
-
-
-
-
-
1
-
-
1
-
4
-
1
-
2
-
1
1
-
-
-
-
-
-
-
-
441227
Kuo
Enzymatic synthesis of deoxy-5 ...
Xanthomonas oryzae
Nature
263
615
1976
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1
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1
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1
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1
3
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1
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1
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1
3
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