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Literature summary for 2.1.1.53 extracted from

  • Biastoff, S.; Teuber, M.; Zhou, Z.S.; Draeger, B.
    Colorimetric activity measurement of a recombinant putrescine N-methyltransferase from Datura stramonium (2006), Planta Med., 72, 1136-1141.
    View publication on PubMed

Application

Application Comment Organism
analysis convenient and accurate enzyme-coupled colorimetric PMT assay, based on the conversion of S-adenosyl-L-homocysteine by 5'-methylthioacenosine/S-adenosylhomocysteine nucleosidase and S-ribosylhomocysteine lyase Datura stramonium

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta-gami (DE3), transformed with a pET-21d vector encoding pmt Datura stramonium

Inhibitors

Inhibitors Comment Organism Structure
S-adenosyl-L-homocysteine
-
Datura stramonium

Organism

Organism UniProt Comment Textmining
Datura stramonium
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by Ni2+-chelate affinity chromatography Datura stramonium

Storage Stability

Storage Stability Organism
4°C, glycine-NaOH buffer, pH 9, stable for 1 month Datura stramonium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + putrescine
-
Datura stramonium S-adenosyl-L-homocysteine + N-methylputrescine
-
?

Synonyms

Synonyms Comment Organism
PMT
-
Datura stramonium

pH Range

pH Minimum pH Maximum Comment Organism
8 9
-
Datura stramonium