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Literature summary for 2.1.1.53 extracted from

  • Walton, N.J.; Peerless, A.C.J.; Robins, R.J.; Rhodes, M.J.C.; Boswell, H.D.; Robins, D.J.
    Purification and properties of putrescine N-methyltransferase from transformed roots of Datura stramonium L (1994), Planta, 193, 9-15.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
2-(aminomethyl)pyridine
-
Datura stramonium
3-methylcadaverine
-
Datura stramonium
4-(aminomethyl)pyridine
-
Datura stramonium
cadaverine
-
Datura stramonium
additional information monoamines p.e. cyclohexylamine, exo-2-aminonorborane, n-butylamine, 4-methylcyclohexylamine, cyclopentylamine; not: 1,3-diaminopropane, 2-(2-aminoethyl)pyridine, 2-aminopyridine, 3-aminopyridine, 4-aminopyridine Datura stramonium
N-methylputrescine
-
Datura stramonium
Na2SO4
-
Datura stramonium
S-adenosyl-L-homocysteine competitive inhibitor Datura stramonium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
S-adenosyl-L-methionine
-
Datura stramonium
0.31
-
putrescine
-
Datura stramonium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
gel filtration Datura stramonium
360000
-
SDS-PAGE Datura stramonium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + putrescine Datura stramonium enzyme catalyzes the initial reaction in the biosynthesis of tropane and pyrrolidine alkaloids from putrescine S-adenosyl-L-homocysteine + N-methylputrescine
-
?

Organism

Organism UniProt Comment Textmining
Datura stramonium
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Datura stramonium

Source Tissue

Source Tissue Comment Organism Textmining
root
-
Datura stramonium
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Datura stramonium

Storage Stability

Storage Stability Organism
-20°C, 12.5% glucose for at least a week Datura stramonium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information requirement for substrate activity: two amino groups in a trans conformation, separated by four carbon atoms Datura stramonium ?
-
?
additional information substrate with little activity: 1,4 -diamino-cis-but-2-ene, not: aliphatic monoamines, 1,3-diaminopropane, 1,5-diaminopentane, N,N`-dimethyl-1,4-diamino-trans-but-2-ene, 1,3-diamino-2-hydroxypropane, 1,3-diaminoacetone, 1,4-diaminobut-2-yne, N-carbamoylputrescine Datura stramonium ?
-
?
S-adenosyl-L-methionine + 1,4-diamino-2,3-dibromo-trans-but-2-ene
-
Datura stramonium S-adenosyl-L-homocysteine + ?
-
?
S-adenosyl-L-methionine + 1,4-diamino-2-hydroxybutane
-
Datura stramonium S-adenosyl-L-homocysteine + ?
-
?
S-adenosyl-L-methionine + 1,4-diamino-2-oxobutane
-
Datura stramonium S-adenosyl-L-homocysteine + ?
-
?
S-adenosyl-L-methionine + 1,4-diamino-trans-but-2-ene
-
Datura stramonium S-adenosyl-L-homocysteine + ?
-
?
S-adenosyl-L-methionine + 4-(aminomethyl)piperidine
-
Datura stramonium S-adenosyl-L-homocysteine + ?
-
?
S-adenosyl-L-methionine + putrescine best substrate Datura stramonium S-adenosyl-L-homocysteine + N-methylputrescine
-
?
S-adenosyl-L-methionine + putrescine enzyme catalyzes the initial reaction in the biosynthesis of tropane and pyrrolidine alkaloids from putrescine Datura stramonium S-adenosyl-L-homocysteine + N-methylputrescine
-
?
S-adenosyl-L-methionine + trans-1,4-diamonocyclohexane
-
Datura stramonium S-adenosyl-L-homocysteine + ?
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
pI: 5.3 Datura stramonium
9
-
-
Datura stramonium

pH Stability

pH Stability pH Stability Maximum Comment Organism
9.5
-
20% loss of activity Datura stramonium
9.8
-
35% loss of activity Datura stramonium

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.01
-
S-adenosyl-L-homocysteine
-
Datura stramonium
0.04
-
cadaverine
-
Datura stramonium
0.15
-
N-methylputrescine
-
Datura stramonium