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Literature summary for 2.1.1.53 extracted from

  • Mizusaki, S.; Tanabe, Y.; Noguchi, M.; Tamaki, E.
    Phytochemical studies on tobacco alkaloids XIV, the occurrence (1971), Plant Cell Physiol., 12, 633-640.
No PubMed abstract available
Search for more literature for 2.1.1.53

Activating Compound

Activating Compound Comment Organism Structure
cysteine slight activatory effect Nicotiana tabacum

General Stability

General Stability Organism
2-mercaptoethanol stabilizes Nicotiana tabacum

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 0.1 mM, strong Nicotiana tabacum
p-chloromercuribenzoate strong Nicotiana tabacum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
 S-adenosyl-L-methionine
-
 Nicotiana tabacum
0.4
-
 putrescine
-
 Nicotiana tabacum

Metals/Ions

Metals/Ions Comment Organism Structure
additional information Mg2+, Mn2+ and EDTA at 0.001 M have no influence Nicotiana tabacum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 gel filtration Nicotiana tabacum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + putrescine Nicotiana tabacum enzyme is probably involved in nicotine biosynthesis S-adenosyl-L-homocysteine + N-methylputrescine
-
 ?

Organism

Organism UniProt Comment Textmining
Nicotiana tabacum
-
 Bright Yellow
-

Purification (Commentary)

Purification (Comment) Organism
-
 Nicotiana tabacum

Source Tissue

Source Tissue Comment Organism Textmining
root roots of decapitated plants show highest activity, no activity in leaves Nicotiana tabacum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.051
-
 putrescine Nicotiana tabacum

Storage Stability

Storage Stability Organism
-20°C, 0.05 M phosphate buffer, pH 7.5, 0.01 M 2-mercaptoethanol, 12.5% glucose, stable for several weeks Nicotiana tabacum
0°C, without addition of a sulfhydryl compound, most activity is lost within a few days Nicotiana tabacum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + N-methylputrescine about 8% of activity compared to putrescine Nicotiana tabacum S-adenosylhomocysteine + N,N-dimethylputrescine
-
 ?
S-adenosyl-L-methionine + putrescine
-
 Nicotiana tabacum S-adenosyl-L-homocysteine + N-methylputrescine
-
 ?
S-adenosyl-L-methionine + putrescine enzyme is probably involved in nicotine biosynthesis Nicotiana tabacum S-adenosyl-L-homocysteine + N-methylputrescine
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
26
-
 assay at Nicotiana tabacum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 9
-
 Nicotiana tabacum

Cofactor

Cofactor Comment Organism Structure
additional information no requirement of cofactors Nicotiana tabacum