General Stability | Organism |
---|---|
divalent cations and 2-mercaptoethanol do not stabilize during purification | Homo sapiens |
substrates stabilize during purification | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3,3-Dimethylbutyrate | competitive to betaine | Homo sapiens | |
Isovalerate | competitive to betaine | Homo sapiens | |
additional information | not inhibitory: methionine or ethionine; product inhibition | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
betaine | - |
Homo sapiens | |
0.12 | - |
L-homocysteine | - |
Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Homo sapiens | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
amino acid composition | Homo sapiens |
45000 | - |
6 * 45000, SDS-PAGE | Homo sapiens |
270000 | - |
gel filtration | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
two forms of the enzyme | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
Storage Stability | Organism |
---|---|
-20°C, many months in 50% v/v glycerol | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N,N,N-trimethylglycine + L-homocysteine | - |
Homo sapiens | N,N-dimethylglycine + L-methionine | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 45000, SDS-PAGE | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
significant heat stability | Homo sapiens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
above, 4 h stable at 37°C | Homo sapiens |