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Literature summary for 2.1.1.45 extracted from

  • Islam, Z.; Gurevic, I.; Strutzenberg, T.S.; Ghosh, A.K.; Iqbal, T.; Kohen, A.
    Bacterial versus human thymidylate synthase Kinetics and functionality (2018), PLoS ONE, 13, e0196506 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.45

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0025
-
 dUMP pH 7.5, 22°C Homo sapiens
0.0029
-
 5,10-methylenetetrahydrofolate presence of Mg2+, pH 7.5, 22°C Homo sapiens
0.0042
-
 dUMP presence of Mg2+, pH 7.5, 22°C Homo sapiens
0.045
-
 5,10-methylenetetrahydrofolate pH 7.5, 22°C Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
additional information presence of Mg2+ does not enhance reaction rates and has no impact on the transition state structure of hydride transfer Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P04818
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methylenetetrahydrofolate + dUMP
-
 Homo sapiens dihydrofolate + dTMP
-
 ?

Synonyms

Synonyms Comment Organism
TYMS
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.52
-
 5,10-methylenetetrahydrofolate presence of Mg2+, pH 7.5, 22°C Homo sapiens
0.54
-
 5,10-methylenetetrahydrofolate pH 7.5, 22°C Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
120
-
 dUMP pH 7.5, 22°C Homo sapiens
120
-
 5,10-methylenetetrahydrofolate presence of Mg2+, pH 7.5, 22°C Homo sapiens
180
-
 dUMP presence of Mg2+, pH 7.5, 22°C Homo sapiens
220
-
 5,10-methylenetetrahydrofolate pH 7.5, 22°C Homo sapiens