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Literature summary for 2.1.1.45 extracted from

  • Jason, T.L.; Figueredo, R.; Ferguson, P.J.; Vincent, M.D.; Berg, R.W.; Koropatnick, J.
    ODN 491, a novel antisense oligodeoxynucleotide that targets thymidylate synthase, exerts cell-specific effects in human tumor cell lines (2008), DNA Cell Biol., 27, 229-240.
    View publication on PubMed
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Inhibitors

Inhibitors Comment Organism Structure
5-fluorodeoxyuridine
-
 Homo sapiens
raltitrexed
-
 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
HeLa cell
-
 Homo sapiens
-
HT-29 cell
-
 Homo sapiens
-
MCF-7 cell
-
 Homo sapiens
-
MDA-MB-231 cell
-
 Homo sapiens
-
MSTO-211H cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methylenetetrahydrofolate + 6-fluoro-dUMP
-
 Homo sapiens ?
-
 ?
5,10-methylenetetrahydrofolate + dUMP
-
 Homo sapiens dihydrofolate + dTMP
-
 ?

Cofactor

Cofactor Comment Organism Structure
N5,N10-methylenetetrahydrofolate
-
 Homo sapiens

Expression

Organism Comment Expression
Homo sapiens thymidylate synthase antisense oligodeoxynucleotide (ODN) 491 decreases thymidylate protein levels in HeLa cells with a concomitant increase in sensitivity to thymidylate synthase-targeting chemotherapeutics. In MCF-7 cells, ODN491 treatment is less effective at reducing thymidylate synthase mRNA and does not reduce thymidylate synthase protein, nor does it enhance sensitivity to thymidylate synthase-targeting or other chemotherapeutics. Treatment with either ODN 83 or ODN 491 reduces 6-fluoro-dUMP binding in HeLa cells by 59% and 62%, respectively, compared with control down