Cloned (Comment) | Organism |
---|---|
cloned and overexpressed in Escherichia coli | Lacticaseibacillus casei |
plasmid pSCTS9, synthetic TS gene in pUC18 | Lacticaseibacillus casei |
Crystallization (Comment) | Organism |
---|---|
- |
Lacticaseibacillus casei |
Protein Variants | Comment | Organism |
---|---|---|
T155C/E188C/C244T | formation of 2 disulfide crosslinks across the subunit interface results in dramatic stabilization of the overall structure of the protein, retention of secondary structure at temperatures as high as 90°C, engineering of an intersubunit crosslink yields a fully active enzyme | Lacticaseibacillus casei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-fluoro-2'-deoxyuridylic acid | - |
Lacticaseibacillus casei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
2 * 35000, SDS-PAGE | Lacticaseibacillus casei |
36620 | - |
calculated molecular mass based on the sequence | Lacticaseibacillus casei |
71000 | - |
analytical ultracentrifugation | Lacticaseibacillus casei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + dUMP | Lacticaseibacillus casei | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Tequatrovirus T4 | - |
dihydrofolate + dTMP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lacticaseibacillus casei | - |
- |
- |
Tequatrovirus T4 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + dUMP | - |
Lacticaseibacillus casei | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Lacticaseibacillus casei | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Tequatrovirus T4 | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Tequatrovirus T4 | dihydrofolate + dTMP | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Tequatrovirus T4 |
dimer | 2 * 35000, SDS-PAGE | Lacticaseibacillus casei |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
mutant enzyme, 2 disulfide bridges across the dimer interface of the enzyme engineered | Lacticaseibacillus casei |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 70 | mutant enzyme, 2 disulfide bridges across the dimer interface of the enzyme engineered | Lacticaseibacillus casei |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
52 | - |
wild-type enzyme, precipitates at 52°C | Lacticaseibacillus casei |
90 | - |
mutant enzyme remains soluble, enhancement of activity is observed up to 60°C, possesses appreciable enzymatic activity at 65°C and retains secondary structure even at 90°C | Lacticaseibacillus casei |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000005 | - |
5-fluoro-2'-deoxyuridylic acid | - |
Lacticaseibacillus casei |