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Literature summary for 2.1.1.45 extracted from

  • Zhang, H.; Cisneros, R.J.; Deng, W.; Zapf, J.W.; Johnson, L.F.; Dunlap, R.B.
    Purification and characterization of recombinant mouse thymidylate synthase (1991), Biochim. Biophys. Acta, 1077, 35-46.
    View publication on PubMed

Application

Application Comment Organism
medicine
-
Mus musculus
medicine
-
Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
-
Homo sapiens
expression vector pETSM, cloned and expressed in Escherichia coli BL21(DE3)pLysS Mus musculus

General Stability

General Stability Organism
glycerol and Triton X-100 stabilize the enzyme Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
5-fluoro-2'-deoxyuridylic acid
-
Homo sapiens
5-fluoro-2'-deoxyuridylic acid
-
Lacticaseibacillus casei
5-fluoro-2'-deoxyuridylic acid
-
Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5,10-methylenetetrahydrofolate + dUMP Mus musculus key function in DNA synthesis, repair and cell division dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP Homo sapiens key function in DNA synthesis, repair and cell division dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP Lacticaseibacillus casei key function in DNA synthesis, repair and cell division dihydrofolate + dTMP
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
human
-
Lacticaseibacillus casei
-
-
-
Mus musculus
-
L1210 cells
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Mus musculus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3
-
affinity chromatography Mus musculus
1.33
-
-
Mus musculus

Storage Stability

Storage Stability Organism
-20°C, activity remains unchanged after 1 month Mus musculus
-20°C, in presence of 0.1% Triton X-100 and 10% glycerol, no loss of activity when stored in lysis buffer for several weeks Mus musculus
4°C, in presence of 0.1% Triton X-100 and 10% glycerol, no loss of activity when stored in lysis buffer for at least 2 weeks Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methylenetetrahydrofolate + dUMP
-
Mus musculus dihydrofolate + dTMP
-
r
5,10-methylenetetrahydrofolate + dUMP
-
Homo sapiens dihydrofolate + dTMP
-
r
5,10-methylenetetrahydrofolate + dUMP
-
Lacticaseibacillus casei dihydrofolate + dTMP
-
r
5,10-methylenetetrahydrofolate + dUMP key function in DNA synthesis, repair and cell division Mus musculus dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP key function in DNA synthesis, repair and cell division Homo sapiens dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP key function in DNA synthesis, repair and cell division Lacticaseibacillus casei dihydrofolate + dTMP
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Mus musculus

pH Range

pH Minimum pH Maximum Comment Organism
4 9
-
Mus musculus