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Literature summary for 2.1.1.45 extracted from

  • Blakeley, R.L.
    The biosythesis of thymidylic acid. IV. Further studies on thymidylate synthetase (1963), J. Biol. Chem., 238, 2113-2118.
No PubMed abstract available
Search for more literature for 2.1.1.45

Activating Compound

Activating Compound Comment Organism Structure
2,3-Dimercaptopropanol absolute requirement for a thiol, synthetase shows greatly dimished activity in absence Escherichia coli
2,3-Dimercaptopropanol absolute requirement for a thiol, synthetase shows greatly dimished activity in absence Homo sapiens
2,3-Dimercaptopropanol absolute requirement for a thiol, synthetase shows greatly dimished activity in absence Enterococcus faecalis
KCN
-
 Escherichia coli
KCN
-
 Homo sapiens
KCN
-
 Enterococcus faecalis

Inhibitors

Inhibitors Comment Organism Structure
5-fluoro-2'-deoxyuridine 5'-(p-aminophenyl phosphate)
-
 Enterococcus faecalis
5-fluoro-2'-deoxyuridylic acid very strong inhibition, pseudo-noncompetitive Enterococcus faecalis
5-fluoro-2'-deoxyuridylic acid
-
 Escherichia coli
5-fluoro-2'-deoxyuridylic acid
-
 Homo sapiens
dihydrofolate
-
 Enterococcus faecalis
dihydrofolate
-
 Escherichia coli
dTMP
-
 Enterococcus faecalis
methotrexate
-
 Homo sapiens
thymidine phosphate product inhibition Enterococcus faecalis
thymidine phosphate
-
 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0012
-
 2'-deoxyuridylate
-
 Escherichia coli
0.0057
-
 2'-deoxyuridylate
-
 Enterococcus faecalis
0.016
-
 methylenetetrahydrofolate
-
 Enterococcus faecalis
0.018
-
 methylenetetrahydrofolate
-
 Enterococcus faecalis
0.035
-
 methylenetetrahydrofolate
-
 Enterococcus faecalis
0.037
-
 methylenetetrahydrofolate
-
 Enterococcus faecalis
0.039
-
 5,10-methylenetetrahydrofolate
-
 Enterococcus faecalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5,10-methylenetetrahydrofolate + dUMP Escherichia coli key function in DNA synthesis, repair and cell division dihydrofolate + dTMP
-
 ?
5,10-methylenetetrahydrofolate + dUMP Homo sapiens key function in DNA synthesis, repair and cell division dihydrofolate + dTMP
-
 ?
5,10-methylenetetrahydrofolate + dUMP Enterococcus faecalis key function in DNA synthesis, repair and cell division dihydrofolate + dTMP
-
 ?
5,10-methylenetetrahydrofolate + dUMP Escherichia coli biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism dihydrofolate + dTMP
-
 ?
5,10-methylenetetrahydrofolate + dUMP Homo sapiens biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism dihydrofolate + dTMP
-
 ?
5,10-methylenetetrahydrofolate + dUMP Enterococcus faecalis biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism dihydrofolate + dTMP
-
 ?

Organism

Organism UniProt Comment Textmining
Enterococcus faecalis
-
 synonym Enterococcus faecalis, strain R
-
Escherichia coli
-
-
-
Homo sapiens
-
 human
-

Purification (Commentary)

Purification (Comment) Organism
partially Homo sapiens
partially Enterococcus faecalis

Source Tissue

Source Tissue Comment Organism Textmining
thymus
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methylene-5,6,7,8-tetrahydrofolate + dUMP
-
 Escherichia coli 7,8-dihydrofolate + dTMP
-
 ?
5,10-methylene-5,6,7,8-tetrahydrofolate + dUMP
-
 Homo sapiens 7,8-dihydrofolate + dTMP
-
 ?
5,10-methylene-5,6,7,8-tetrahydrofolate + dUMP
-
 Enterococcus faecalis 7,8-dihydrofolate + dTMP
-
 ?
5,10-methylenetetrahydrofolate + dUMP reverse reaction is at least 630times slower than the forward reaction Escherichia coli dihydrofolate + dTMP
-
 r
5,10-methylenetetrahydrofolate + dUMP reverse reaction is at least 630times slower than the forward reaction Homo sapiens dihydrofolate + dTMP
-
 r
5,10-methylenetetrahydrofolate + dUMP reverse reaction is at least 630times slower than the forward reaction Enterococcus faecalis dihydrofolate + dTMP
-
 r
5,10-methylenetetrahydrofolate + dUMP key function in DNA synthesis, repair and cell division Escherichia coli dihydrofolate + dTMP
-
 ?
5,10-methylenetetrahydrofolate + dUMP key function in DNA synthesis, repair and cell division Homo sapiens dihydrofolate + dTMP
-
 ?
5,10-methylenetetrahydrofolate + dUMP key function in DNA synthesis, repair and cell division Enterococcus faecalis dihydrofolate + dTMP
-
 ?
5,10-methylenetetrahydrofolate + dUMP biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism Escherichia coli dihydrofolate + dTMP
-
 ?
5,10-methylenetetrahydrofolate + dUMP biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism Homo sapiens dihydrofolate + dTMP
-
 ?
5,10-methylenetetrahydrofolate + dUMP biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism Enterococcus faecalis dihydrofolate + dTMP
-
 ?
additional information
-
 Escherichia coli ?
-
 ?
additional information
-
 Homo sapiens ?
-
 ?
additional information UMP, dCMP and deoxyuridine are no substrates Enterococcus faecalis ?
-
 ?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000004
-
 5-fluoro-2'-deoxyuridylic acid
-
 Enterococcus faecalis
0.023
-
 methotrexate uncompetitive with methylene tetrahydrofolate Homo sapiens
0.024
-
 dTMP
-
 Enterococcus faecalis